Anti-hypercholesterolemic and Antioxidant Potential of Combined Lemon Juice, Ginger Extract, and Apple Cider Vinegar in High-cholesterol Diet-induced Rats | ||
| Egyptian Journal of Botany | ||
| Articles in Press, Accepted Manuscript, Available Online from 18 November 2025 | ||
| Document Type: Regular issue (Original Article) | ||
| DOI: 10.21608/ejbo.2025.369120.3233 | ||
| Authors | ||
| Noor A. Majeed* ; Zuhair R. Addai | ||
| Department of Biology, College of Education for Pure Science, University of Thi-Qar, 64001, Iraq | ||
| Abstract | ||
| Natural lemon juice, apple cider vinegar and ginger rhizome extract are natural food products that are characterized by their delicious taste in addition to their high nutritional value due to their many active compounds and antioxidants. In our current study, we evaluated a mixture of lemon extract, ginger and apple cider vinegar against hypercholesterolemic in male rats. The animals were then randomly assigned to four groups (G1: Normal control, G2: Hypercholesterolemic control, G3: Hypercholesterolemic was treated with a 25mg/mL extract, G4: hypercholesterolemic was treated with a 50mg/mL extract. The lemon juice, ginger extract, and natural apple cider vinegar used in the study were extracted aqueously in a ratio of 25:10:2, respectively. The anti- hypercholesterolemic activity in male rats on a high-cholesterol diet was evaluated by daily dosing of the animals for four weeks at two concentrations of 25mg/mL and 50mg/mL. The free radical scavenging assay was utilized in the determination of the antioxidant activity. Both before and after treatment, body weight was measured. Lipid levels, blood glucose and liver enzymes were measured at the end of the experiment. The groups’ results showed significant differences. The high-cholesterol diet led to an increase in body weight, TC, T.G, LDL, blood sugar, and a decrease in HDL, ALT and AST in the negative control group, while a positive response was observed in G3 and G4 treated with lemon extract, ginger, and natural apple cider vinegar, leading to a decrease in body weight, lipids profile, blood sugar, and a significant increase in liver enzymes. The results confirm that the extracts of lemon, ginger, and apple cider vinegar are potent antioxidants that reduce oxidative stress and lipid levels in the blood of male rats. | ||
| Keywords | ||
| Anti-Hypercholesterolemic; Antioxidant; Cholesterol diet; Edible plants | ||
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