Harnessing Pyocyanin: A Bioactive Metabolite from Pseudomonas aeruginosa with Antimicrobial and Anticancer Potential | ||
| Egyptian Journal of Botany | ||
| Articles in Press, Accepted Manuscript, Available Online from 18 November 2025 | ||
| Document Type: Regular issue (Original Article) | ||
| DOI: 10.21608/ejbo.2025.372233.3251 | ||
| Authors | ||
| Mohamed K. Ibrahim1; Yehia A. El-Zawhry2; Ahmed A. Esmaiel2; Ahmed A. Askora2; Mohga T.A. Mostafa* 1 | ||
| 1Department of Microbiology, Faculty of Science, Ain Shams University, Cairo, Egypt | ||
| 2Department of Microbiology, Faculty of Science, Zagazig University, Zagazig, Egypt | ||
| Abstract | ||
| Pseudomonas aeruginosa is a clinically significant bacterium responsible for both acute and chronic infections. In this study, out of 66 isolates, 46 pigmented isolates, which were obtained from clinical samples, were selected based on their pigmentation on a cetrimide agar. The selected strain, Pseudomonas aeruginosa P73 (ON01478), demonstrated the highest pyocyanin production at a concentration of 13.5µg/mL. The purification of pyocyanin was carried out using thin-layer chromatography (TLC), yielding an Rf value of approximately 0.81. Confirmation of the pigment as pyocyanin was achieved through Fourier-transform infrared spectroscopy (FTIR), UV-visible spectrophotometric analysis, and gas chromatography-mass spectrometry (GC-MS) characterization. The antimicrobial activity of purified pyocyanin was assessed using the well diffusion method. The results indicated that Klebsiella pneumoniae exhibited resistance to pyocyanin’s inhibitory effects, whereas Staphylococcus aureus was found to be sensitive. Pyocyanin also demonstrated an antifungal activity against fungal strains of Aspergillus niger, more sensitive than that against Aspergillus flavus, and also the activity against yeast strains Candida albicans was more sensitive than that against Candida tropicalis. Furthermore, the anticancer potential of pyocyanin was evaluated using the MTT assay against HepG2 (human hepatocarcinoma), A-549 (lung carcinoma), MCF-7 (breast adenocarcinoma), and HCT-116 (colon carcinoma) cell lines. Pyocyanin exhibited promising anticancer effects, with the responsiveness of the cancer cell lines ranked as follows: HepG2 > MCF-7 > HCT-116 > A-549. This study highlights the potential of pyocyanin as both an antimicrobial and anticancer agent. | ||
| Keywords | ||
| Anticancer effects; Antimicrobial properties; Purification; Pyocyanin | ||
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