Cassia auriculata Flower Extract-mediated Gold Nanoparticles and Their Antimicrobial, Antioxidant, and Potential in vitro Anticancer Activity on PA-1 Ovarian Cancer Cells | ||
| Egyptian Journal of Botany | ||
| Articles in Press, Accepted Manuscript, Available Online from 18 November 2025 | ||
| Document Type: Regular issue (Original Article) | ||
| DOI: 10.21608/ejbo.2025.377853.3274 | ||
| Authors | ||
| Mamatha R. Seethammagari1; Bharathi Pasupuleti1; Venkata S. Kotakadi* 2; Varadarajulu N. Challagundla3; Josthna Penchalaneni1 | ||
| 1Department of Biotechnology, Sri Padmavathi Mahila University, Tirupati, AP. India | ||
| 2DST-PURSE Centre, Sri Venkateswara University, Tirupati-517502, Andhra Pradesh, India | ||
| 3Department of Biotechnology, Dravidian University, Kuppam, AP. India | ||
| Abstract | ||
| A simple and green method for synthesizing gold nanoparticles (AuNPs) using Cassia auriculata flower extract (CaF) is proposed in this study. The biosynthesized CaF-AuNPs were characterized using various spectroscopy techniques. The characteristic surface plasmon resonance peak was observed at 556nm. Particle size analysis by DLS revealed an average size of 36.3± 1nm with a polydispersity index (PDI) of 0.287, while zeta potential measurements indicated good stability with a value of -24.5mV. SEM and TEM analyses confirmed spherical nanoparticles with sizes ranging from 10–40nm and 5–32nm, respectively, with an average size of 34± 2nm determined by TEM. XRD analysis confirmed the crystalline nature of the AuNPs, and EDAX analysis demonstrated their elemental composition and high purity. The AuNPs exhibited significant antibacterial activity against gram-positive and gram-negative bacteria and noteworthy antioxidant activity in DPPH NO and H₂O₂ assays. Cytotoxicity studies using the MTT assay on PA-1 ovarian cancer cells demonstrated significant anticancer potential with an IC50 value of 30.89µg/mL. Further studies on the Cell cycle and apoptosis using IC50 value concentration revealed exceptional results, suggesting their applicability in biomedical applications. These eco-conscious green synthesized gold nanoparticles have shown various potential therapeutic applications. | ||
| Keywords | ||
| Antibacterial activity; Anticancer activity; Antioxidant activity; CaF-AuNPs; PA-1 Ovarian cancer cell lines; Spectral characterization | ||
|
Statistics Article View: 2 |
||
