Histological Study on The Possible Therapeutic Effect of Mesenchymal Stem Cells Versus Their Derived Exosomes in Carbon Tetrachloride Induced Lung Injury in Adult Male Albino Rat | ||
| Ain Shams Medical Journal | ||
| Articles in Press, Accepted Manuscript, Available Online from 19 November 2025 PDF (4.63 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/asmj.2025.396047.1474 | ||
| Authors | ||
| Faten A. Mahmoud1; Eman Hamdy Eltantawy1; Samar Fekry Ezzat* 2 | ||
| 1Histology Faculty of Medicine Ain Shams University | ||
| 2Histology and cell biology department Faculty of medicine Ain shams University Cairo-Egypt | ||
| Abstract | ||
| Background: Lung fibrosis is a deadly, progressive illness. CCl4 is a xenobiotic used to cause lung fibrosis. Mesenchymal stem cells (MSCs) garnered interest as a possible therapeutic option. Research has demonstrated exosomes' (EXOs) potential in tissue regeneration. Aim: Evaluate the possible therapeutic effects of mesenchymal stem cells versus their derived exosomes on induced lung fibrosis in rat. Material: 46 adult male rats were used. 10 rats were used for preparation of MSCs and isolation of exosomes. 36 Rats were separated into 4 groups, Group I (control) n=15, Group II (n=7): received intraperitoneal injection (IP) of 30% CCl4 of 3 ml/kg body weight in olive oil twice weekly for 8 weeks. Group III (n=7): received CCl4 like group II. Then each rat received 1×10⁶ MSCs in 150μL PBS once through the tail vein at the beginning of week 9. Group IV (n=7): received CCl4 like group II. Then received 150 μg of EXOs diluted in 150μL PBS once through tail vein at the beginning of week 9. At week 10, rats were scarified. Lung specimens underwent histological, morphometric, and statistical analysis. Results: CCL4 led to mononuclear infiltration, congested blood capillaries, extravasation, thickening in interalveolar septa and increased deposition of collagen. Disrupted microvilli and empty lamellar bodies were present. MSCs & EXOs improved the previous histological changes. However, EXOs resulted in a significant improvement Conclusion: Both MSCs and EXOs had significant effects in improving CCL4 induced injury in the lung, but EXOs were better. | ||
| Keywords | ||
| Exosomes; Lung fibrosis; MSCs; rat | ||
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