Optimization and bioactive compounds production by Streptomyces griseus to resist Staphylococcus aureus isolated from human semen | ||
| Biological and Biomedical Journal | ||
| Volume 3, Issue 2, July 2025, Pages 264-275 PDF (611.34 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bbj.2025.414736.1127 | ||
| Authors | ||
| Ayat Saied* 1; Eman A Hassan1; Hany Sabry1; Zeinab K Abd El-Aziz2 | ||
| 1International Islamic Center for Population Studies and Research, Al-Azhar University. | ||
| 2Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt. | ||
| Abstract | ||
| The purpose of this work was to optimize the conditions under which Streptomyces griseus might effectively develop its antibacterial capabilities against resistant Staphylococcus aureus. In this work, S. griseus was grown at various incubation periods, different pH levels, various incubation temperatures, different agitation rates, different media, and various nitrogen sources. The yield in each case was extracted and tested versus previously isolated resistant S. aureus, which was isolated from semen and had a correlation with infertility. The optimal conditions for the cultivation of S. griseus to produce extract with maximal antibacterial efficacy were recorded. The chemical composition of the extract was tested using GC-MS. The antioxidant capability of the extract was determined using DPPH. Furthermore, the extract cytotoxicity versus Vero cells was examined via MTT assay. The results revealed that cultivation of S. griseus on starch nitrate at 3 days for incubation, pH 7.0, 30°C, 150 rpm, and addition of soybean as a nitrogen source resulted in maximal production of antimicrobial extract of S. griseus with a zone of inhibition of 4.62±0.42 cm, and MIC was detected at 125±0.35 µg/ml. The extract had 39 various compounds and antioxidant effect (IC50 2.45±0.21 µg/ml), and minimal cytotoxicity with IC50 192.97 ± 2.64 µg/ml. Thus, S. griseus is an intriguing supplier of novel antimicrobials, according to this study. In order to find powerful antibacterial agents against resistant infections, the synthesis of bioactive chemicals was successfully increased by combining statistical optimization, antimicrobial evaluation, and culture condition improvement. | ||
| Keywords | ||
| Actinomycetes; Antioxidant; GC-MS; Resistant bacteria Streptomyces griseus; Staphylococcus aureus | ||
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