Isolation and Molecular Characterization of Lactobacillus johnsonii Isolate for its Application as an Immunomodulatory Probiotic in Chicken | ||
| Egyptian Journal of Veterinary Sciences | ||
| Articles in Press, Corrected Proof, Available Online from 23 November 2025 PDF (996.37 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejvs.2025.416620.3073 | ||
| Authors | ||
| mahmoud Abdelmongy1; aya abdelmoez* 2; nasser sherif2 | ||
| 1Microbial Biotechnology Department, Genetic Engineering and Biotechnology Researches Institute, University of Sadat City, Egypt | ||
| 2Central Laboratory for Evaluation of Veterinary Biologics (CLEVB). Agricultural Research Center (ARC), Abbasia, Cairo, Egypt. | ||
| Abstract | ||
| This investigation aimed to isolate and characterize particular microorganisms from the intestinal tract of chickens that could function as promising probiotic agents for poultry. Probiotics exert direct influences on the digestive system while indirectly affecting the immune response in chickens. Intestinal samples were obtained from 25 disease-free chickens aged 21 days at a facility located at the Central Laboratory for Evaluation of Veterinary Biologics (CLEVB), Cairo, Egypt. Following the isolation of 25 bacterial strains, their identity was confirmed through DNA analysis. One particular strain was identified as Lactobacillus johnsonii through its cultivation on MRS medium and biochemical profile analysis. Molecular identification using PCR methodology was performed to confirm the Lactobacillus johnsonii species, subsequently validated through 16S rRNA genetic sequencing and phylogenetic evaluation. The identified strain was registered in GenBank under accession code PV616976 L. johnsonii. The results of this investigation validate the effective isolation and identification of an indigenous Lactobacillus johnsonii strain from the chicken digestive tract, demonstrating its capability as an advantageous probiotic agent for improving poultry health and immune function. | ||
| Keywords | ||
| chickens; immunity; intestine; sequencing | ||
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