DEVELOPMENT AND EVALUATION OF A RABBIT POLYCLONAL ANTIBODIES-BASED ELISA FOR THE DETECTION OF BOVINE PREGNANCY-ASSOCIATED GLYCOPROTEIN (PAG) AS A DIAGNOSTIC TOOL FOR BOVINE PREGNANCY | ||
| Journal of the Egyptian Veterinary Medical Association | ||
| Volume 84, Issue 1, April 2024, Pages 77-85 PDF (313.17 K) | ||
| Document Type: Original Research Articles | ||
| Abstract | ||
| The aim of this study was to develop a polyclonal antibody (Rabbit anti-BovPAG) capable of detecting PAG in the serum of pregnant cows. Five rabbits were immunized against synthetic peptide bovine PAG (BovPAG) preparations. Each 0.5 ml of PAG protein (1000μg) was reconstituted in 500μl Phosphate Buffer Saline (PBS) and emulsified in equal volume with complete and incomplete Freund's adjuvant.The mixture of immunizer and adjuvant was injected subcutaneously at multiple sites along the dorsal area of the rabbits. Blood samples were collected from the marginal ear vein, starting before the first injection (Baseline) and every 14 days after that. Rabbit anti-BovPAG levels were measured using indirect ELISA. It can be concluded that synthetic peptide bovine PAG (BovPAG) is a suitable source for producing specific rabbit anti-BovPAG antibodies and has the potential for development as a key antigen for antibody production as a base for pregnancy diagnosis in bovine kits. | ||
| Keywords | ||
| ELISA; BovPAG; Polyclonal Antibody; Rabbitanti-BovPAG | ||
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