Effects of Various Concentrations of Autologous Platelets-Rich Plasma on Cutaneous Wound Healing in Experimental Rabbit Model | ||
| Egyptian Journal of Veterinary Sciences | ||
| Articles in Press, Corrected Proof, Available Online from 24 November 2025 PDF (775.08 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejvs.2025.365370.2807 | ||
| Authors | ||
| Fawad Khalil Pitafi1; Hamid Akbar* 2; Muhammad Abid Hayat3; Ayesha Safdar4; Aneela Zameer Durrani5; Ghulam Mustafa6; Muhammad Talha Sajjad7; Abdul Asim Farooq8 | ||
| 1Department of Veterinary Surgery, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences, Lahore, Pakistan | ||
| 2Department of Veterinary Surgery and Pets Sciences,Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences, Lahore, Pakistan | ||
| 3School of medicine, Jiangsu University | ||
| 4Department of Veterinary Surgery, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences, Lahore 54000, Pakistan | ||
| 5Faculty of Veterinary sciences, University of Veterinary and Animal Sciences, Lahore, Pakistan | ||
| 6Department of Veterinary Pathology, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences 54000, Lahore, Pakistan | ||
| 7Department of Veterinary Surgery and Pet Sciences, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences 54000, Lahore, Pakistan | ||
| 8Department of veterinary Surgery, Faculty of Veterinary sciences, University of Veterinary and Animal sciences Lahore Pakistan | ||
| Abstract | ||
| This study explored the effect of various concentrations of PRP on cutaneous wound healing in rabbits. Twenty-four rabbits allocated randomly into four groups: group-A (control), group-B (0.2 mL PRP), group-C (0.4 mL PRP), and group-D (0.6 mL PRP) with 6 rabbits in each group. In each rabbit, 2 cm from the midline, full-thickness (2 x 2 cm2) skin incisions were made on the right dorsal surface. Re-epithelization and neovascularization were assessed on day 21 by H&E staining, while collagen formation were assessed by Mason’s trichrome staining. The serum MDA concentrations and CAT activity were also assessed using blood samples. The groups that received 0.4 mL and 0.6 mL of PRP (subcutaneously on days 1, 7, and 14) had significantly higher levels of re-epithelization, angiogenesis, fibroblasts, and collagen fibers arrangements than the control group (irrigated with sterile saline) on day 21. The 0.2 mL PRP group also had a significant increase in collagen arrangement on day 21. The both groups C & D wounds had significantly lower MDA levels on day 7, and they were highly significantly lower on days 14 and 21 than the control wound. However, the group B wound was lower on days 14 and 21 than the control wound. The CAT activity increased non-significantly on different time intervals between the groups. In conclusion, 0.4 mL and 0.6 mL PRP-groups accelerated wound healing by promoting re-epithelization, neovascularization, collagen organization, and inhibiting oxidative stress than the control wounds. | ||
| Keywords | ||
| cutaneous wounds; histopathology; PRP; rabbits; wound healing | ||
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