Amentoflavone attenuates Indomethacin-Induced Gastric Ulcer in Rats by up-regulation of Nrf2/HO-1 signaling pathway | ||
| Medicine Updates | ||
| Articles in Press, Accepted Manuscript, Available Online from 25 November 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/muj.2025.438351.1282 | ||
| Authors | ||
| Mohamed A Zayed1; Suzan A Khodir* 1; Mona El-Said Farag2; Noha Abd El-aziz3; Heba Mostafa El Hagary4; Maha Tarek Mohammed5; Rofanda M Bakeer6; Reem Safwat Mohamed Zein7; Asmaa I Bayomi8; Waleed Mahmoud Ragab9; Khaled Mohamed Elsadek10; Mona Mohamed Ahmed11 | ||
| 1Medical Physiology Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia, Egypt. | ||
| 2Medical Physiology Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia, Egypt | ||
| 3Anatomy and Embryology Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia, Egypt | ||
| 4Tropical Medicine Department, Faculty of Medicine, Menoufia University | ||
| 5Clinical Pharmacology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt | ||
| 6Pathology Department , Faculty of medicine Helwan University, Cairo, Egypt, Pathology Department, Saint Petersburg university, Cairo , Egypt | ||
| 7Medical Biochemistry & Molecular Biology Department, Faculty of Medicine, Benha University | ||
| 8Zoology department, Faculty of Science, Menoufia University. | ||
| 9Anatomy and Embryology Department, Faculty of Medicine, Fayoum University, Fayoum, Egypt, Anatomy and Embryology Department, Faculty of Medicine, Galala University, Attaka, Suez, Egypt | ||
| 10Pathology Department, Damietta Faculty of Medicine, Al-Azhar University, Damietta, Egypt | ||
| 11Department of Physiology, Faculty of medicine, Cairo University, Cairo, Egypt, Biophysiology Department, Ibn Sina National College for Medical Studies, Jeddah, Saudi Arabia. | ||
| Abstract | ||
| Background: One of common gastrointestinal conditions in the world is peptic ulcers. Indomethacin (IND) has a higher tendency to cause ulcers. Amentoflavone (AMF) has anti-inflammatory, anti-apoptotic, and antioxidant qualities. Objective: The goal of the study was to asses the gastroprotective impact of AMF in IND-induced stomach ulcers and underlying processes. Material and Methods: Thirty male albino rats were divided into: control, Ulcer, and Ulcer+ AMF. Gastric ulcer index values, gastric PH, gastric PGE2, gastric MDA, gastric SOD, gastric TNF-α, gastric IL-1β, gastric IL-10, and gastric Nrf2 and HO-1 gene expression were assessed. Histopathologic and NF-kB and Bax immunohistochemical analyses of the gastric tissue were also carried out. Results: compared to control, the ulcer group exhibited significantly higher gastric ulcer index values, gastric MDA, gastric TNF-α, gastric IL-1β, and gastric NF-kB and Bax immunoreaction, while gastric PH values, gastric SOD, gastric IL-10, and gastric PGE2, as well as gastric gene expression of Nrf2 and HO-1 were dramatically decreased. AMF significantly reduced the alterations in stomach tissue brought on by IND. Conclusion: Through its antioxidant, anti-inflammatory, and anti-apoptotic qualities and upregulating the Nrf2/HO-1 signaling pathway, AMF exhibits gastroprotective benefits in rats with IND-induced ulcers. | ||
| Keywords | ||
| Amentoflavone. Gastric Ulcer; HO-1. Indomethacin; NF-kB. Nrf2 | ||
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