Luteolin attenuates Doxorubicin-Induced Cardiac Toxicity in Rats by downregulation of TLR4/NF-kB signaling pathway | ||
| Medicine Updates | ||
| Articles in Press, Accepted Manuscript, Available Online from 25 November 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/muj.2025.439045.1283 | ||
| Authors | ||
| Karima El-Sayed1; Suzan A Khodir* 2; Ghada S Amer3; Noha Abd El-aziz4; Nouran K Olama5; Sara A Khedr6; Walaa M El gherriany7; Mohamed Mahmoud Abdellah8; Samah M. Abdelkhalek9; Asmaa I Bayomi10; Mona Mohamed Ahmed11 | ||
| 1Medical Physiology Department, Faculty of Medicine, Suez Canal University, Ismailia 41522, Egypt | ||
| 2Medical Physiology Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia, Egypt. | ||
| 3Medical Physiology Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia, Egypt | ||
| 4Anatomy and Embryology Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia, Egypt | ||
| 5Anatomy and Embryology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt | ||
| 6Clinical Pharmacology Department, Faculty of Medicine, Ain Shams university, Egypt. | ||
| 7Internal Medicine Department, Haematology Unit, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia, Egypt. | ||
| 8Pathology Department, Faculty of Medicine, Fayoum University, Egypt, Pathology Department , Galala University, Suez, Egypt | ||
| 9Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Benha University, Egypt | ||
| 10Zoology department, Faculty of Science, Menoufia University. | ||
| 11Department of Physiology, Faculty of medicine, Cairo University, Cairo, Egypt, Biophysiology Department, Ibn Sina National College for Medical Studies, Jeddah, Saudi Arabia. | ||
| Abstract | ||
| Background: the use of doxorubicin (Dox), anticancer antibiotic, has been restricted due to its potential toxicity to critical organs, such as the heart. Strong anti-inflammatory and anti-oxidant properties are exhibited by luteolin (Lut). Aim of the study: to demonstrate the cardiac protective effect of Lut in DOX-induced cardiotoxicity and the underlying processes. Material and methods: Three groups of thirty male albino rats were created: DOX, DOX+Lut, and control (10/group). Serum cTnI, serum LDH, serum CK-MB, serum lipid, cardiac MDA, cardiac SOD, cardiac TNF-α, cardiac IL-6, cardiac IL-10, cardiac TLR4 gene expression, and cardiac NF-kB gene expression were all measured. Cardiac immunohistochemistry analyses were also carried out. Results: Serum cTnI, serum LDH, serum CK-MB, serum cholesterol, serum triglycerides, cardiac MDA, cardiac TNF-α, cardiac IL-6, cardiac gene expression of TLR4 and NF-kB, and cardiac caspase-3 & NF-kB immunoreaction were all substantially higher in the DOX than in the control, while decreased cardiac index value, cardiac SOD, and cardiac IL-10. Lut substantially reduced the cardiac alterations brought on by DOX. Conclusion: By downregulating the cardiac TLR4/NF-kB pathway and exhibiting anti-inflammatory, antiapoptotic, antioxidant, and lipid-lowering properties, Lut has a cardio-protective effect in DOX rats. | ||
| Keywords | ||
| Cardiotoxicity. Caspase-3; Doxorubicin. Luteolin; NF-kB. TLR4 | ||
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