Supplementation of Cattle Follicular Fluid Improves Developmental Competence of Buffalo Oocytes | ||
| Egyptian Journal of Veterinary Sciences | ||
| Articles in Press, Corrected Proof, Available Online from 25 November 2025 PDF (827.44 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejvs.2025.425557.3148 | ||
| Authors | ||
| Muhammad Usman Mehmood* 1; Nabila Gulzar2; Liping Pu3; Zahid Naseer4; Kehuan Lu3 | ||
| 1Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning, China & Department of Theriogenology, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences, Lahore, Pakistan | ||
| 2Department of Dairy Technology, University of Veterinary and Animal Sciences, Lahore, Pakistan | ||
| 3Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning, China. | ||
| 4Faculty of Veterinary and Animal Sciences, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi-46000, Pakistan | ||
| Abstract | ||
| During in vitro embryo production (IVEP), follicular fluid acts as a natural supplement to the maturation medium; however, species differences in its effectiveness remain unclear. The study aimed to compare progesterone and estradiol concentrations in cattle and buffalo follicular fluid from the dominant follicle and to assess the effect of cattle follicular fluid on the developmental competence of buffalo oocytes. Buffaloes and cows were synchronized using CIDR-based protocols, and follicular fluid was aspirated from the dominant follicle (≥ 12 mm) of slaughtered ovaries. Estradiol and progesterone concentrations were quantified in the follicular fluid, which was then incorporated into the IVM medium, divided into three groups: Cattle follicular fluid (cFF), Buffalo follicular fluid (buFF), and control containing oocytes (n = 1073) retrieved from 668 ovaries of slaughtered buffaloes. The results indicated that cFF exhibited higher estradiol (E2), progesterone (P4), and estradiol/progesterone ratio compared to buFF aspirated from dominant follicles. Buffalo oocytes supplemented with cFF exhibited higher maturation rates based on cumulus expansion, compared to other treatments. Supplementation of cFF into the maturation media improved the cleavage, morula, blastocyst rates, as well as the cell count of hatched blastocysts, compared to the other treatments. In conclusion, incorporating cFF into the maturation medium enhanced cumulus expansion and improved the developmental competence of buffalo oocytes, leading to higher blastocyst yield. | ||
| Keywords | ||
| Cattle follicular fluid; Cumulus expansion; Buffalo; Synchronization; Developmental competence | ||
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