Molecular Genetic Studies on the Effect of Carica Papaya linn Seeds Extract Against Colon Cancer Induced by Dimethylhydrazine in Male rats | ||
| SVU-International Journal of Veterinary Sciences | ||
| Articles in Press, Accepted Manuscript, Available Online from 25 November 2025 | ||
| Document Type: Research article | ||
| DOI: 10.21608/svu.2025.414791.1424 | ||
| Authors | ||
| Nadia S Mahrous1; Hala Kamal Tagian* 2; Abdel Albaset M Ebied3 | ||
| 1Department of Zoology , Faculty of Science, South Valley University | ||
| 2Zoology Department, Faculty of Science South Valley University,Nagi Hamdy, Qena, Egypt | ||
| 3Zoology Department, Faculty of Science South Valley University , Qena, Egypt | ||
| Abstract | ||
| Colorectal cancer is the third most prevalent cancer globally and the second leading cause of cancer deaths. Studies indicate that papaya (Carica papaya Linn) seeds have anti-cancer characteristics that could be beneficial against multiple kinds of cancer, such as colon cancer. The research explored the impact of Carica papaya Linn seed extract against colorectal cancer. Subcutaneous injections of dimethylhydrazine (DMH) (10 mg/kg) were employed to stimulate colon cancer in rats. The tumor-bearing rats with ten rats in each group were divided into three treatment groups, each receiving different doses of papaya seed extract (3, 9, and 15 ml/kg). Finally, whole animals were killed, then tissue samples were obtained to evaluate alterations in TP53, CASP3, APC, COX2, and BCL2 genes through RT-PCR. Additionally, measuring the protein levels through ELISA and analyzing the cell cycle profile using flow cytometry. Our findings revealed that papaya seed extract induced cytotoxicity, enhanced programmed cell death and necrosis in tumor cells, and significantly reduced tumor size. Papaya seed extract exhibits strong anti-cancer effects against colon cancer, highlighting its potential as a therapeutic selection. | ||
| Keywords | ||
| Keywords: Colorectal cancer; DMH; Carica papaya Linn; RT-PCR; ELISA | ||
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