Selective Cytotoxic and Chemoprotective effect of nanocurcumin against human HCT-116 cell line | ||
| Biochemistry Letters | ||
| Article 7, Volume 14, Issue 1, 2018, Pages 85-93 PDF (734.51 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/blj.2018.47586 | ||
| Authors | ||
| Youssef M. Shehata1; Nermin Rafaat2; Haytham A. Gad3; Sara F. Gaafar4 | ||
| 1Professor of Biochemistry, Faculty of Veterinary Medicine, Zagazig University | ||
| 2Assistant Professor of Medical Biochemistry, Faculty of Medicine, Zagazig University. | ||
| 3Assistant Professor of Biochemistry, Faculty of Veterinary Medicine, Zagazig University. | ||
| 4B.V. SC., Diploma in Biochemistry, Faculty of Veterinary Medicine, Zagazig University. | ||
| Abstract | ||
| Background: limitation of curcumin use as antioxidant and anticancer may related to its low bioavailability so its use as nanocurcumin may improve its effect. Aim: this study was designed to investigate the possible chemoprotective and cytotoxic effect of nanocurcumin against Human colorectal carcinoma (HCT-116) cell line Material and methods: MTT assay was performed to detect the cytotoxic effect of nanocurcumin against normal and HCT-116 cells, and determination of gene expression of P53, BAX, Caspase-9 and BCL-2 was performed to detect the apoptotic effect of nanocurcumin Results: nanocurcumin has a selective cytotoxic effect on human HCT-116 cells with no cytotoxic effect on healthy cells. At the same time, nanocurcumin increases the expression of P53, BAX and Caspase-9 with reduction in expression of BCL-2 gene in medium and high doses with no effect in lower dose Conclusion: nanocurcumin has selective cytotoxic and chemoprotective effect against human HCT-116 cells according to its dose used. | ||
| Keywords | ||
| colorectal cancer; HCT-116; Nanocurcumin; MTT assay; p53; Bax; Bcl2; caspase-9 | ||
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