A Phylogeny of Envelope protein like gene of Lumpy skin disease virus, Egypt, 2014 | ||
| Benha Veterinary Medical Journal | ||
| Article 29, Volume 34, Issue 3, August 2018, Pages 338-354 PDF (1.28 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bvmj.2018.47868 | ||
| Authors | ||
| G.HE.M El-kady.; G.F. El-Bagoury; E.M. El-Nahas; A.S. El-Habbaa | ||
| Virology Department, Faculty of Veterinary Medicine, Benha University, Egypt. | ||
| Abstract | ||
Numerous of Cases of skin nodules eruptions tied to lumpy skin disease virus (LSDV) are becoming more frequent in Qailubia Province, Egypt amongst cattle populations in 2014. In this study, a strain of LSDV (LSDV-Elkady-2014) associated with that outbreak was isolated from scabs of clinically infected Frisian cattle on chorioallantoic membrane (CAM) of specific pathogen free embryonated chicken eggs (SPF-ECEs). The isolate induced hemorrhage, enlarged liver, slight heart hypertrophy of chick embryos, with a pock lesion on CAM. Identification of LSDV-Elkady-2014 isolate was confirmed by histopathology, electron microscopy (EM), immunoflourescence (IF), and polymerase chain reaction (PCR) targeting LSDV envelope protein-like gene. Based on the partial sequence analysis of LSDV envelope protein-like gene. LSDV strain was phylogenetically related to LSDV- NI-2490, LSDV/ NW/LW, and LSDV/2/slemani/Kurdistan/ 2014. In conclusion, LSDV is still circulated in Egypt, and the isolated strain induced characteristic properties on chick embryos that were not previously recorded by LSDV. | ||
| Keywords | ||
| LSDV; chick embryo; EM; Phylogeny; Egypt | ||
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