Identification of Novel Polyphenolic Secondary Metabolites from Pistacia Atlantica Desf. and Demonstration of their Cytotoxicity and CCl4 induced Hepatotoxicity in Rat | ||||
| Egyptian Journal of Chemistry | ||||
| Article 10, Volume 63, Issue 1, January 2020, Page 117-130 PDF (622.82 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejchem.2019.16297.1994 | ||||
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| Authors | ||||
Sahar Awad Allah Hussein   1; Amani M.D. El-Mesallamy2; Salma O.K. Othman3; Abd el-Mohsen M. Soliman4
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| 1Head of department of Phytochemistry and Plant Systematics,Division of Pharmaceutical Industries,National Research Center, El Buhouth st., Dokki Dokki, Cairo, Egypt | ||||
| 2Department of Chemistry, Faculty of Science, Zagazig University, Zagazig, Egypt | ||||
| 3Department of Chemistry, Faculty of Science, Sebha University, Sebha, Libya | ||||
| 4Department of Therapeutic Chemistry; National Research Centre, Cairo, Egypt | ||||
| Abstract | ||||
| As of late a great deal of studies have been led to distinguish regular mixes for counteractive action of the advancement and repeat of malignant growths. The present investigation went for investigating the auxiliary metabolites substance of Pistacia atlantica Desf. (Anacardiaceae) leaves extracts and surveying their cytotoxic activity towards some malignant growth cell lines. In addition, the defensive impact of aqueous methanol and ethyl acetate extracts towards the CCl4 induced hepatotoxicity in rodents was explored. Novel components isolation was done utilizing customary chromatographic systems. The structures of the novel components were clarified dependent on the UV, NMR spectral data information alongside their mass-spectrometric investigations. The ethyl acetate extract of P. atlantica leaves contains a complicated mixture of phenolic acids and gallotannines, were elucidated for the first time from this plant , including polyphenolic acid; ellagic acid (1); 3,3’- dimethoxyellagicacid (2) and gallotannines, namely: 1,2,3,4,6-penta-O-galloyl-β-4C1-glucopyranose (3); 1,6-digalloylglucopyranose (4) ;1,3-digalloyl glucopyranose (5); 2,3-digalloyl-glucopyranose,nilocitin (6) ;2,3,6-trigalloylglucopyranose (7) and 2,3-di-O-galloyl-4,6-O-hexahydroxydiphenoyl-(/)-4C1-glucopyranose, (8) . The identification of isolated compounds by conventional methods, spectroscopic analysis, including 1D-NMR , 2D-NMR, ESIMS and HRESI mass as well. The search for novel, potentially biologically active extract becomes much more efficient after identification of all compounds in that mixture. In vitro cytotoxic activity of methanol and ethyl acetate extracts of Pistacia atlantica and resulted new compounds on four human cancer cell lines to be specific: Colorectal adenocarcinoma cell line (Caco-2 cell line), Prostate carcinoma cell line ( PC3 cell line), hepatocellular carcinoma (HEPG2), Caucasian bosom adenocarcinoma (MCF-7). SRB assay was used to measure the potential cytotoxicity. The ethyl acetate extract showed a higher cytotoxicity to Caco-2 cell line with IC50 = 3.38 µg/ml and PC3 cell line with IC50=14.3 µg/ml. Furthermore, the methanol extract was least cytotoxic to normal cell lines. The strong cytotoxic potential was observed in pure compound pentagalloyl glucopyranose (3) to all three cancer cell lines (HEPG2, Caco-2, MCF-7) , IC50 of HEPG2 value = 4.5 µg/ml. the IC50 for Caco-2 was 11 µg/ml. and MCF7, IC50=13.5 µg/ml. as well , in comparison with pure compounds (4,7,8). The growth inhibition of 50% (IC50) for each extract was calculated from the optical density of treated and untreated cells. | ||||
| Keywords | ||||
| Keywords: Pistacia Atlantica Desf. (Anacardiaceae); Novel polyphenolic compounds; in vitro cytotoxic activity; CCl4; Hepatoprotective activity | ||||
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