Acaricidal Activity of Essential Oils of Chamomile, Marjoram and Eucalyptus against the Two-Spotted Spider Mite, Tetranychus urticae Koch: Biology and Enzymes | ||||
Acarines: Journal of the Egyptian Society of Acarology | ||||
Article 3, Volume 3, Issue 1, January 2009, Page 9-15 PDF (146.47 K) | ||||
Document Type: Original research articles | ||||
DOI: 10.21608/ajesa.2009.4960 | ||||
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Authors | ||||
A. Afify1; Fatma Ali2; M. Mohamed3; A. Turky2 | ||||
1Biochemistry Dept., Fac. of Agric., Cairo Univ. | ||||
2Zoology and Nematology Dept., Fac. of Agric., Cairo Univ. | ||||
3Biochemistry Dept., Fac. of Agric., Cairo Univ., | ||||
Abstract | ||||
Different concentrations of three essential oil extracts (0.5, 1, 2, 3 and 4%) were used against Tetranychus urticae. (Koch) Chamomile; Chamomilla recutita (L.), proved to be the most efficient agent against T. urticae followed by Marjoram; Marjorana hortensis (L.), and Eucalyptus (Eucalyptus sp.) The LC50 values of these oils were 0.651 &1.17; 1, 84 & 6.26 and 2.18 & 7.33 for adults and eggs, respectively. Herein, the bioassay experiments of the two essential oils, Chamomile and Marjoram showed relationship between essential oil contents and activity of enzyme glutathione S-transferase, non specific esterase and alkaline phosphatase in T.urticae. GC-MS analysis of C. recutita and M. hortensis proved the presence of 13 and 14 essential oil components, respectively. The major essential oil contents of C. recutita are α-Bisabolol oxide A (35.25%), and Trans-β-farersene (7.98%), while the main components of M. hortensis are Terpinen-4-ol (23.86%), P-cymene (23.40%) and Sabinene (10.90%). The major components of both plant extracts (Terpinen-4-ol 23.86% and α-Bisabolol oxide A 35.25% essential oils) may be responsible for the activity of enzymes of T. urticae (glutathione S-transferase, non specific esterase and alkaline phosphatase). | ||||
Keywords | ||||
Tetranychidae; Plant essential oils; Enzymes; Glutathione S-transferase; Non specific esterase and Alkaline phosphatase | ||||
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