High expression of the checkpoint molecule PD-1 on conventional CD4+ and regulatory T cells in the peripheral blood of Hepatocellular carcinoma patients
Salem, M., Elokeily, H., ElShourbgy, I., Elwan, N. (2018). High expression of the checkpoint molecule PD-1 on conventional CD4+ and regulatory T cells in the peripheral blood of Hepatocellular carcinoma patients. EKB Journal Management System, 2(2), 11-18. doi: 10.21608/jcbr.2018.37777
Mohamed Salem; Hanan Abd ElGhany Elokeily; Ibrahim ElShourbgy; Nadia Elwan. "High expression of the checkpoint molecule PD-1 on conventional CD4+ and regulatory T cells in the peripheral blood of Hepatocellular carcinoma patients". EKB Journal Management System, 2, 2, 2018, 11-18. doi: 10.21608/jcbr.2018.37777
Salem, M., Elokeily, H., ElShourbgy, I., Elwan, N. (2018). 'High expression of the checkpoint molecule PD-1 on conventional CD4+ and regulatory T cells in the peripheral blood of Hepatocellular carcinoma patients', EKB Journal Management System, 2(2), pp. 11-18. doi: 10.21608/jcbr.2018.37777
Salem, M., Elokeily, H., ElShourbgy, I., Elwan, N. High expression of the checkpoint molecule PD-1 on conventional CD4+ and regulatory T cells in the peripheral blood of Hepatocellular carcinoma patients. EKB Journal Management System, 2018; 2(2): 11-18. doi: 10.21608/jcbr.2018.37777

High expression of the checkpoint molecule PD-1 on conventional CD4+ and regulatory T cells in the peripheral blood of Hepatocellular carcinoma patients

Egyptian Journal of Cancer and Biomedical Research
Article 3, Volume 2, Issue 2 - Serial Number 3, August 2018, Page 11-18  XML PDF (2.65 MB)
Document Type: Original Article
DOI: 10.21608/jcbr.2018.37777
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Authors
Mohamed Salem email orcid 1; Hanan Abd ElGhany Elokeily email 2; Ibrahim ElShourbgy2; Nadia Elwan3
1Immunology and Biotechnology Division, Department of Zoology, Faculty of Science, Tanta University, Egypt
2Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt
3Department of Tropical Medicine, Faculty of Medicine, Tanta University, Tanta, Egypt
Abstract
Background: Regulatory T cells (Tregs), possess a suppression function leading to T cells exhaustion and tumor progression through serious of signaling pathways of inhibitory receptors under pathological conditions in particular cancer including hepatocellular carcinoma. Conventional helper CD4+ T cells can be converted into regulatory T cells that may suppress anti HCC immunity through different mechanisms including expression of PD-1. PD-1 inhibitory checkpoint and its ligand PDL-1 emerge immune escape through interaction between T cell and tumor microenvironment. Aim: The aim of this study is to analyze the expression level of the regulatory checkpoint (PD-1) on T cells in HCC patients. Materials and methods: The numbers of conventional and regulatory T cells and their expression of checkpoint receptor PD-1 were analyzed in the peripheral blood of HCC patients (n=20) as well as healthy control volunteers (n=15) using multi-parametric flow cytometry after staining with anti-CD4, anti-CD25, anti-CD127 for (T cells) and anti PD-1 (CD279). Results: As compared to healthy control volunteers HCC patients showed high relative numbers of Tregs expressing CD4+CD25+CD127- and decrease relative number of conventional CD4+ defines as CD4+CD25-CD127-. These cells showed high expression by increasing level of PD-1 where CD4+ showed high expression than Tregs. Similar cells number of PD-1 expression where observed at the level of absolute numbers. Conclusion: HCC patients before treatment express high level of the checkpoint molecule PD-1 opening the door for further investigations.
Keywords
Cancer; CD4; Checkpoint; Hepatocellular carcinoma; Immune; PD-1; Regulatory T cells
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