The effect of melatonin on apoptosis in hepatocellular carcinoma cell line: An experimental study | ||||
Medical Journal of Viral Hepatitis | ||||
Article 6, Volume 3.2, Issue 2, April 2019, Page 75-79 PDF (211.53 K) | ||||
Document Type: Original article | ||||
DOI: 10.21608/mjvh.2019.55733 | ||||
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Authors | ||||
Elsaadawy Sara1; Elbadawy Reda2; Abd Elaziz Badawy2; Mosaad Ghadeer2; Elkareem Heba3 | ||||
1Resident of Gastroenterology & Hepatology dept, Damietta Cardiology and Gastroenterology center Egypt. | ||||
2Hepatology, Gastroenterology & Infectious Diseases dept., Benha Univ., Egypt. | ||||
3Medical Biochemistry dept., Faculty of Medicine, Benha Univ., Egypt. | ||||
Abstract | ||||
Introduction: Apoptosis resistance in Hepatocellular Carcinoma (HCC) is considered as one of the most important factors involved in hepatocarcinogenesis and its progression. Recent experimental research of melatonin highlights its anticancer function through its antioxidant effect, induction of apoptosis and cell cycle arrest, inhibition of metastasis, and suppression of angiogenesis. The aim of our study is to explore the effect of melatonin on casp 8 mRNA expression and Alpha-fetoprotein (AFP) mRNA expression in HepG2 HCC cell line by using real time PCR technique to proof its apoptotic effect. Methods: HepG2 cells were treated with 75mg melatonin then AFP mRNA expression and casp8 expression is investigated by RT PCR. Results: Melatonin significantly increased caspase 8 expression. The relative expression was 2.51, 2.48 and1.89 respectively as compared to control (P <0.001). While the expression of AFP was down regulated by melatonin administration. The relative expression of AFP was 0.051, 0.052 and 0.046 respectively as compared to control (P <0.001). Conclusion: melatonin decreases AFP gene expression and stimulates apoptosis via up regulation of caspase 8 expression in HCC. Melatonin can be considered as promising adjuvant for chemotherapy in HCC. | ||||
Keywords | ||||
Alpha fetoprotein (AFP); Hepatocellular carcinoma (HCC); apoptosis; Melatonin | ||||
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