Genetic Diversity in Wheat Genotypes Using Random Amplified Polymorphic DNA (RAPD) | ||||
| Assiut Journal of Agricultural Sciences | ||||
| Article 5, Volume 46, Issue 2, April 2015, Page 50-70 PDF (120.93 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ajas.2015.558 | ||||
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| Abstract | ||||
| Genetic diversity among 75 wheat genotypes (66 hexaploids and 9 tetraploids) was studied using 6 RAPD primers. A total of 76 DNA bands were generated by the 6 RAPD primers from all genotypes, of which 53 (70.23%) were polymorphic. The total number of amplification products per primer ranged from 6 (OPO06) to 16 (UBC03 and UBC09) with an average of 12.67 bands per primer. The size of PCR products ranged from 1478bp (OPA01) to 91bp (UBC03). A significant correlation (0.892, p<0.01) was observed between the total number of bands and the number of polymorphic bands. The six primers amplified 11 unique bands for specific genotypes that would prove to be highly useful in genotype identification and designing the future breeding strategy. The similarity coefficient values ranged from 0.68 to 1.00, indicating moderate to high genetic similarityin tested loci among the wheat genotypes. The 75 wheat genotypes were grouped into two major clusters within the dendrogram. Cluster I had the tetraploid genotypes and the hexaploid genotypes were placed in cluster II. | ||||
| Keywords | ||||
| Wheat; RAPD; Genetic diversity; PCR; varietal identification | ||||
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