Nematicidal Effect of A Wild Type of Serratia Marcescens and Its Mutants Against Meloidogyne Incognita Juveniles
Kassab, S., Eissa, M., Badr, U., Ismail, A., Abdel Razik, A., Soliman, G. (2017). Nematicidal Effect of A Wild Type of Serratia Marcescens and Its Mutants Against Meloidogyne Incognita Juveniles. EKB Journal Management System, 16(2), 95-114. doi: 10.21608/ejaj.2017.56204
S. Kassab; M. Eissa; U. Badr; A. Ismail; A. Abdel Razik; Gaziea Soliman. "Nematicidal Effect of A Wild Type of Serratia Marcescens and Its Mutants Against Meloidogyne Incognita Juveniles". EKB Journal Management System, 16, 2, 2017, 95-114. doi: 10.21608/ejaj.2017.56204
Kassab, S., Eissa, M., Badr, U., Ismail, A., Abdel Razik, A., Soliman, G. (2017). 'Nematicidal Effect of A Wild Type of Serratia Marcescens and Its Mutants Against Meloidogyne Incognita Juveniles', EKB Journal Management System, 16(2), pp. 95-114. doi: 10.21608/ejaj.2017.56204
Kassab, S., Eissa, M., Badr, U., Ismail, A., Abdel Razik, A., Soliman, G. Nematicidal Effect of A Wild Type of Serratia Marcescens and Its Mutants Against Meloidogyne Incognita Juveniles. EKB Journal Management System, 2017; 16(2): 95-114. doi: 10.21608/ejaj.2017.56204

Nematicidal Effect of A Wild Type of Serratia Marcescens and Its Mutants Against Meloidogyne Incognita Juveniles

Egyptian Journal of Agronematology
Article 4, Volume 16, Issue 2, July 2017, Page 95-114  XML PDF (247.01 K)
Document Type: Original Article
DOI: 10.21608/ejaj.2017.56204
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Authors
S. Kassab1; M. Eissa2; U. Badr3; A. Ismail2; A. Abdel Razik3; Gaziea Soliman2
1Department of Plant Protection, Fac. of Agric., Ain Shams Univ., Shoubra El-Kheima, Cairo, Egypt.
2Nematology Lab., Plant Pathology Department, National Research Centre, Dokki, Giza, Egypt
3Department of Genetic and Cytology, National Research Centre, Dokki, Giza, Egypt
Abstract
Serratia marcescens (SM) is currently considered as a biocontrol agent
against plant parasitic nematodes. It is one of the most effective bacteria for
degradation of chitin. This lytic bacteria was evaluated on the survival of
Meloidogyne incognita juveniles under laboratory conditions. The best treatment by
(SM 36) mutant achieved zero viable juveniles in either S or S/10 i.e, highly
effective on juveniles mortality compared to the wild type of SM and untreated
control which recorded 51.8, 49.3 and 49 juveniles, respectively. The numbers of
non viable juveniles of the best treatment were 6, 4 and 2.8 individuals after 24, 48
and 72 hrs, respectively compared to the SM wild type which achieved 26.3. 33.3
and 27.3 while the untreated control did not show any effect on the juveniles. There
were positive relationships between the nematode mortality and each of the
bacteria concentration and enzyme production from the mutants. The numbers of
either viable juveniles or non viable juveniles were reduced at S or S/10 dilutions
and exposure periods of 24, 48 and 72 hr compared to the untreated control. This
reduction was attributed to the dead nematode bodies which were degraded and
destroyed by these bacterial mutants. Mutation achieved increasing of chitinase and
alkaline protease-over producing mutants, which produced two to three times more
endochitinase activity than the wild type of S.marcescens.

Keywords
lytic bacteria; Chitinase; Protease; Serratia marcescens; Mutation; Meloidogyne incognita
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