Study of different genotypes of Vitamin-D Receptor (VDR) Gene Polymorphisms in Patients Eligible for Allogeneic Hematopoietic Stem Cell Transplantation (Allo-HSCT) from a Sibling Donor | ||||
| SECI Oncology Journal | ||||
| Article 1, Volume 4, Issue 3, July 2016, Page 35-39 PDF (180.23 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/secioj.2016.5682 | ||||
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| Abstract | ||||
| Background: Vitamin-D receptor (VDR) is found in both normal and neoplastic hemopoietic cells, and VDR gene polymorphism may play an important role in the clinical outcome of HLA-matched sibling donor allogeneic hematopoietic stem cell transplantation (allo-HSCT). The distribution of the genotypes in a given population would affect the impact of the polymorphism in different conditions including the clinical outcome of HSCT. Objectives: to study the different genotypes of VDR polymorphisms in patients eligible for sibling donor alloHSCT. Patients and methods: Eighty-one patients undergoing allo-HSCT and their HLA-identical sibling donors were examined using polymerase chain reaction for restriction fragment length polymorphism (PCR-RFLP) analysis for FokI (rs10735810), ApaI (rs7975232) and TaqI (rs731236) polymorphisms. Results: Patients’ genotype frequencies of the three VDR polymorphisms were as follows: for FokI: Heterozygous (H) 76/78 (97.4%), mutant (M) 1/78 (1.3%) and wild (W) 1/78 (1.3%); for ApaI: (H) 26/72 (36.1%), (M) 7/72 (9.7%), and (W) 39/72 (54.2%); for TaqI: (H) 37/68 (54.4%) and (W) 31/68 (45.6%). While donors’ genotype frequencies were as follows: for FokI: (W) 40/75 (53.4%), (H) 28/75 (37.3%) and (M) 7/75 (9.3%); for ApaI: (W) 31/66 (47.0%), (H) 28/66 (42.4%) and (M) 7/66 (10.6%); for TaqI: (W) 26/65 (40.0%), (H) 38/65 (58.5%) and (M) 1/65 (1.5%). Conclusion: The genotype frequencies of VDR polymorphisms in our population were different from that of Caucasians and Asian countries, and this variability of distribution could explain that there is ethnic variability in vitamin D receptor gene polymorphisms. | ||||
| Keywords | ||||
| Vitamin-D Receptor (VDR); FokI (rs10735810); ApaI (rs7975232); TaqI (rs731236); hematopoietic stem cell transplantation (HSCT) | ||||
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