MiRNA-200b as a prognostic factor in invasive duct carcinoma | ||||
| SECI Oncology Journal | ||||
| Article 1, Volume 3, Issue 3, March 2015, Page 69-75 PDF (203.87 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/secioj.2015.5700 | ||||
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| Abstract | ||||
| Background: miR-200b has been reported to be a tumor suppressor and a promising therapeutic target in cancer. miR-200b has been associated with epithelial- mesenchymal transition (EMT) and chemo-resistance in cancer. Aim of work: To determine the expression of miR-200b in breast cancer, invasive duct carcinoma (IDC) tissues in comparison to normal adjacent breast tissues. Also, the prognostic role of miR-200b expression in breast cancer patients was evaluated. Material and Methods: Quantitative real time polymerase chain reaction (qRT-PCR) was used to detect the expression level of miR-200 b in 48 tumor tissues and 14 adjacent normal tissues from breast cancer patients with IDC. Results: The expression of miR-200b in breast cancer tissues was significantly lower than in normal tissues (p < 0.005). In comparison with the normal tissues, miR-200b was down regulated in 75% (36 /48) of the tumor samples. Low expression of miR - 200b were insignificantly higher in patients with distant metastases (75% vs 25%), vascular invasion (71.1% vs 28.9%), estrogen receptor expression (77.8% vs 22.2%), progesterone receptor expression (84 % vs 16%) and lymph nodes metastasis (72.2% vs 27.8%) in comparison to those with high expression of miR- 200b. Conclusion: Reduced miR- 200b expression is a frequent event in human breast cancer tissues and could be involved in breast cancer carcinogenesis. miR-200b could be a prognostic factor in breast cancer patients. | ||||
| Keywords | ||||
| invasive duct carcinoma; miRNA-200b; qRT-PCR | ||||
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