IMMUNOHISTOCHEMICAL EXPRESSION OF TLR-4 AND ITS CORRELATION TO VARIOUS GRADES OF ORAL SQUAMOUS CELL CARCINOMA
Omar, E., Omar, T., El-Sheikh, S., Riad, S., Mourad, G., Afifi, M. (2018). IMMUNOHISTOCHEMICAL EXPRESSION OF TLR-4 AND ITS CORRELATION TO VARIOUS GRADES OF ORAL SQUAMOUS CELL CARCINOMA. EKB Journal Management System, 43(3), 60-64. doi: 10.21608/adjalexu.2018.57995
Enas M. Omar; Taissir A. Omar; Sahar M. El-Sheikh; Sahar E. Riad; Ghada M. Mourad; Marwa M. Afifi. "IMMUNOHISTOCHEMICAL EXPRESSION OF TLR-4 AND ITS CORRELATION TO VARIOUS GRADES OF ORAL SQUAMOUS CELL CARCINOMA". EKB Journal Management System, 43, 3, 2018, 60-64. doi: 10.21608/adjalexu.2018.57995
Omar, E., Omar, T., El-Sheikh, S., Riad, S., Mourad, G., Afifi, M. (2018). 'IMMUNOHISTOCHEMICAL EXPRESSION OF TLR-4 AND ITS CORRELATION TO VARIOUS GRADES OF ORAL SQUAMOUS CELL CARCINOMA', EKB Journal Management System, 43(3), pp. 60-64. doi: 10.21608/adjalexu.2018.57995
Omar, E., Omar, T., El-Sheikh, S., Riad, S., Mourad, G., Afifi, M. IMMUNOHISTOCHEMICAL EXPRESSION OF TLR-4 AND ITS CORRELATION TO VARIOUS GRADES OF ORAL SQUAMOUS CELL CARCINOMA. EKB Journal Management System, 2018; 43(3): 60-64. doi: 10.21608/adjalexu.2018.57995

IMMUNOHISTOCHEMICAL EXPRESSION OF TLR-4 AND ITS CORRELATION TO VARIOUS GRADES OF ORAL SQUAMOUS CELL CARCINOMA

Alexandria Dental Journal
Article 10, Volume 43, Issue 3, December 2018, Page 60-64  XML PDF (820.39 K)
Document Type: Original Article
DOI: 10.21608/adjalexu.2018.57995
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Authors
Enas M. Omar1; Taissir A. Omar2; Sahar M. El-Sheikh2; Sahar E. Riad3; Ghada M. Mourad4; Marwa M. Afifi5
1Master in Oral Pathology Department, Faculty of Dentistry, Alexandria University
2Professor in Oral Pathology Department, Faculty of Dentistry, Alexandria University
3. Professor in Oral Pathology Department, Faculty of Dentistry, Alexandria University
4Professor in Histology and Cell Biology Department, Faculty of Medicine, Alexandria University.
5Lecturer in Oral Pathology Department, Faculty of Dentistry, Alexandria University
Abstract
INTRODUCTION: Oral cancer is a major health problem, causing high morbidity and mortality rates. Oral squamous cell carcinoma (OSCC) accounts for 90-95% of all oral malignancies. During the last decade, significant evidence has suggested that inflammation plays an important role in tumorigenesis, and microenvironment-derived signals which are important constituents in all tumors. Toll-like receptors (TLRs) are classical signals that play a key role in the innate immune system. They drive the inflammatory response and control the cellular proliferation and survival by stimulating the immune cells and promoting integrated processes of inflammation and tissue repair. This can predict that TLR4 may be of great significance in tumors. Hence, this study is to evaluate and correlate the expression of TLR-4 in various grades of oral squamous cell carcinoma (OSCC). OBJECTIVES: To evaluate and correlate the expression of TLR-4 in different histopathological grades of OSCC MATERIALS AND METHODS: An immunohistochemical (IHC) study using the TLR-4 antibody were done on 50 surgical specimens and 10 normal mucosal tissues taken from OSCC patients and healthy individuals, respectively. The staining were performed using a Labeled Strept-Avidin Biotin complex method (LSAB) RESULTS: TLR-4 was expressed in human OSCC biopsies and the expression level was correlated with the tumor differentiation. Higher expression was detected in the well and the moderately differentiated squamous cell carcinomas than the poorly differentiated ones. CONCLUSIONS: TLR-4 expression could be a prognostic factor in oral squamous cell carcinoma cases as expression was detected in the well and moderately differentiated higher than poorly differentiated squamous cell carcinomas.
Keywords
Oral squamous cell carcinoma; TLR-4
References
  1. Bray F, Ferlay J, Laversanne M, Brewster DH, Gombe Mbalawa C, Kohler B, et al. Cancer Incidence in Five Continents: Inclusion criteria, highlights from Volume X and the global status of cancer registration. International journal of cancer. 2015;137:2060-71.
  2. Omar K, Camile SF, Newell WJ. Oral and oropharyngeal cancer in the Middle East and North Africa: Incidence, mortality, trends, and gaps in public databases as presented to the Global Oral Cancer Forum. Translational Research in Oral Oncology. 2017;2:2057178X17698480.
  3. Choi S, Myers JN. Molecular pathogenesis of oral squamous cell carcinoma: implications for therapy. Journal of dental research. 2008;87:14-32.
  4. Akira S, Takeda K. Toll-like receptor signalling. Nature Reviews Immunology. 2004;4:499.
  5. Rich AM, Hussaini HM, Parachuru VPB, Seymour GJ. Toll-Like Receptors and Cancer, Particularly Oral Squamous Cell Carcinoma. Frontiers in Immunology. 2014;5:464.
  6. Makinen LK, Atula T, Hayry V, Jouhi L, Datta N, Lehtonen S, et al. Predictive role of Toll-like receptors 2, 4, and 9 in oral tongue squamous cell carcinoma. Oral Oncol. 2015;51:96-102.
  7. Mai CW, Kang YB, Pichika MR. Should a Toll-like receptor 4 (TLR-4) agonist or antagonist be designed to treat cancer? TLR-4: its expression and effects in the ten most common cancers. OncoTargets and therapy. 2013;6:1573-87.
  8. Sato Y, Goto Y, Narita N, Hoon DSB. Cancer Cells Expressing Toll-like Receptors and the Tumor Microenvironment. Cancer Microenvironment. 2009;2:205-14.
  9. Vidya MK, Kumar VG, Sejian V, Bagath M, Krishnan G, Bhatta R. Toll-like receptors: Significance, ligands, signaling pathways, and functions in mammals. International Reviews of Immunology. 2018;37:20-36.
  10. Syrbu SI, Cohen MB. An enhanced antigen-retrieval protocol for immunohistochemical staining of formalinfixed, paraffin-embedded tissues. Methods Mol Biol. 2011;717:101-10.
  11. Kotz S, Balakrishnan N, Read CB, Vidakovic B. Encyclopedia of statistical sciences. 2nd ed. Hoboken, N.J.: Wiley-Interscience; 2006.
  12. Kirkpatrick LA, Feeney BC. A simple guide to IBM SPSS statistics for version 20.0. Student ed. Belmont, Calif.: Wadsworth, Cengage Learning; 2013
  13. Ibrahim AS, Khaled HM, Mikhail NN, Baraka H, Kamel H. Cancer incidence in Egypt: Results of the national population-based cancer registry program. J Cancer Epidemiol. 2014;2014:1–18.
  14. Markopoulos AK. Current Aspects on Oral Squamous Cell Carcinoma. The Open Dentistry Journal. 2012;6:126-30.
  15. Scott SE, Grunfeld EA, Main J, McGurk M. Patient delay in oral cancer: a qualitative study of patients' experiences. Psycho-oncology. 2006;15:474-85.
  16. Dajon M, Iribarren K, Cremer I. Dual roles of TLR7 in the lung cancer microenvironment. OncoImmunology. 2015;4:e991615.
  17. Dajon M, Iribarren K, Cremer I. Toll-like receptor stimulation in cancer: A pro- and anti-tumor double-edged sword. Immunobiology. 2017;222:89-100.
  18. van Zyl A, Bunn B. Clinical features of oral cancer. SADJ. 2012;67:566–9.
  19. Mesquita JA, Cavalvanti AL, Nonaka CFW, Godoy GP, Alves PM. Clinical and histopathological evidence of oral squamous cell carcinoma in young patients : systematized review. J Bras Patol Med Lab. 2014;50:67–74.
  20. Pires FR, Ramos AB, de Oliveira JBC, Tavares AS, da Luz PSR, dos Santos TCRB. Oral squamous cell carcinoma: clinicopathological features from 346 cases from a single Oral Pathology service during an 8-year period. J Appl Oral Sci. 2013;21:460–7.
  21. Effiom OA, Adeyemo WL, Omitola OG, Ajayi OF, Emmanuel MM, Gbotolorun OM. Oral squamous cell carcinoma: a clinicopathologic review of 233 cases in Lagos, Nigeria. J Oral Maxillofac Surg. 2008;66:1595–9.
  22. Tandon P, Dadhich A, Saluja H, Bawane S, Sachdeva S. The prevalence of squamous cell carcinoma in different sites of oral cavity at our Rural Health Care Centre in Loni, Maharashtra – a retrospective 10-year study. Contemp Oncol. 2017;21:178–83.
  23. Kotrashetti VS, Nayak R, Bhat K, Hosmani J, Somannavar P. Immunohistochemical expression of TLR4 and TLR9 in various grades of oral epithelial dysplasia and squamous cell carcinoma, and their roles in tumor progression: a pilot study. Biotechnic & histochemistry : official publication of the Biological Stain Commission. 2013;88:311-22.
  24. Sugawara Y, Uehara A, Fujimoto Y, Kusumoto S, Fukase K, Shibata K, et al. Toll-like receptors, NOD1, and NOD2 in oral epithelial cells. Journal of dental research. 2006;85:524-9.
  25. Sun Z, Luo Q, Ye D, Chen W, Chen F. Role of toll-like receptor 4 on the immune escape of human oral squamous cell carcinoma and resistance of cisplatin-induced apoptosis. Molecular cancer. 2012;11:33-.
  26. Ali A, Natah S, Konttinen Y. Differential expression of Toll-like receptors in chronic hyperplastic candidosis. Oral microbiology and immunology. 2008;23:299-307.
  27. Makinen LK, Ahmed A, Hagstrom J, Lehtonen S, Makitie AA, Salo T, et al. Toll-like receptors 2, 4, and 9 in primary, metastasized, and recurrent oral tongue squamous cell carcinomas. Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology. 2016;45:338-45.
  28. Pakdel F , Pouralibaba F , Pakdel S , Khiyavi RK , Falsafi P , Eslami H , et al. Expression of toll-like receptors in squamous cell csrcinoma of the tongue. Archives of Applied Science Research. 2015; 7:20-24
  29. Park J-H, Yoon H-E, Jeon D-I, Ahn S-G, Yoon J-H. Activation of TLR2 and TLR5 did not affect tumor progression of an oral squamous cell carcinoma, YD-10B cells. Journal of Oral Pathology & Medicine. 2010;39:781- 5.
  30. Rydberg C, Månsson A, Uddman R, Riesbeck K, Cardell L-O. Toll-like receptor agonists induce inflammation and cell death in a model of head and neck squamous cell carcinomas. Immunology. 2009;128:e600-e11.
  31. Du B, Jiang Q-L, Cleveland J, Liu B-R, Zhang D. Targeting Toll-like receptors against cancer. Journal of Cancer Metastasis and Treatment. 2016;2:463-70.
  32. Huang L, Xu H, Peng G. TLR-mediated metabolic reprogramming in the tumor microenvironment: potential novel strategies for cancer immunotherapy. Cellular And Molecular Immunology. 2018.
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