BIOLOGICAL ACTIVITY OF MINIMAL DOSE OF AEROSOLIZED INTERFERON-α IN MALE ALBINO RATS | ||
| Research Journal of Applied Biotechnology | ||
| Article 13, Volume 2, Special issue (1) for the first International Conference of Genetic Engineering and Biotechnology, November 2016, Pages 128-133 PDF (626.52 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/rjab.2016.59643 | ||
| Authors | ||
| Khalil A. El-Halfawy1; Shaden M. Hanafy1; Bahgat A. EL-Fiky2; Hassan M. Hassan1 | ||
| 1Molecular Biology Department, Genetic Engineering and Biotechnology Institute, University of Sadat City, Egypt | ||
| 2Animal Biotechnology Department, Genetic Engineering and Biotechnology Institute, University of Sadat City, Egypt. | ||
| Abstract | ||
| buffy coat leukocytes Interferon-α (IFN-α) is a leucocyte-derived cytokine with pleiotropic effects on the cells of the immune system, including the ability to promote viral and microbial killing.Five batches of buffy coat leukocytes IFN-α were prepared and purifiied from five male buffalos under the same conditions of collections ; before,during and after slaughter . Three IFN-α doses; 200.500 and 1000 IU were prepared. The present study was designed to evaluate the biologically active dosage of aerosolized buffy coat leukocytes IFN-α, in male albino rats. Four healthy groups were included: Group 1 (control, n=10) received 0 IFN- α ,Group 2 (n=10) received 200 IU IFN-α , Group 3 received 500 IU and Group(4) received 1000 IU / day in order to determine the minimal IFN-α dose able to induce biological effects without side-effects. Result data showed that 500 IFN- α IU/ day for five days induced a significant biological activity, increasing serum levels of biochemical , hematological and IFN-γ levels. It is concluded that aerosolized IFN-α administration to the lung is well-tolerated at biologically active doses. | ||
| Keywords | ||
| Buffy Coat Leukocyte IFN-α; biochemical; hematological parameters and IFN-γ | ||
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