In vitro evaluation of anti-schistosomal properties of diethyledithiocarbamate sodium salt loaded with chitosan-nanoparticles
El-Bolkiny, Y., Kenawy, E., Al-Shourbagy, I., Basyony, M., Salama, R., Alkhazendar, A. (2019). In vitro evaluation of anti-schistosomal properties of diethyledithiocarbamate sodium salt loaded with chitosan-nanoparticles. EKB Journal Management System, 3(2), 31-40. doi: 10.21608/jcbr.2019.37784
Yousry E El-Bolkiny; El-Refaie Kenawy; Ibrahim Al-Shourbagy; Mohmed Basyony; Reda Salama; Abdelhalim Alkhazendar. "In vitro evaluation of anti-schistosomal properties of diethyledithiocarbamate sodium salt loaded with chitosan-nanoparticles". EKB Journal Management System, 3, 2, 2019, 31-40. doi: 10.21608/jcbr.2019.37784
El-Bolkiny, Y., Kenawy, E., Al-Shourbagy, I., Basyony, M., Salama, R., Alkhazendar, A. (2019). 'In vitro evaluation of anti-schistosomal properties of diethyledithiocarbamate sodium salt loaded with chitosan-nanoparticles', EKB Journal Management System, 3(2), pp. 31-40. doi: 10.21608/jcbr.2019.37784
El-Bolkiny, Y., Kenawy, E., Al-Shourbagy, I., Basyony, M., Salama, R., Alkhazendar, A. In vitro evaluation of anti-schistosomal properties of diethyledithiocarbamate sodium salt loaded with chitosan-nanoparticles. EKB Journal Management System, 2019; 3(2): 31-40. doi: 10.21608/jcbr.2019.37784

In vitro evaluation of anti-schistosomal properties of diethyledithiocarbamate sodium salt loaded with chitosan-nanoparticles

Egyptian Journal of Cancer and Biomedical Research
Article 6, Volume 3, Issue 2 - Serial Number 4, August 2019, Pages 31-40    PDF (2.36 M)
Document Type: Original Article
DOI: 10.21608/jcbr.2019.37784
Authors
Yousry E El-Bolkiny* 1; El-Refaie Kenawy2; Ibrahim Al-Shourbagy3; Mohmed Basyony3; Reda Salama3; Abdelhalim Alkhazendar4
1Department of Zoology, Faculty of Science, Tanta University, Egypt
2Chemistry Department, Faculty of Science, Tanta University, Tanta, Egypt.
3Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt.
4Zoology Department, Faculty of Science, Tanta University
Abstract
Biologically active agents show several biomedical applications. Non-chitosan loaded diethyldithiocarbamate (DDC) was previously found to have anti-schistosomal activity. The present work aimed to prepare DDC-Chitosan-Na-nanoparticles (DDC-Chitosan-Na-NPs), and to investigate it’s in vitro anti-schistosomal activity with slow release approach and as well as its mortality % against both concentrations and exposure time. NPs were prepared by suspension cross linking method, in which 0.16 g chitosan were dissolved in 16 ml 2% acetic acid solution (ratio 1% wt/v.). Then after, 0.1g DDC-Na salt was added to the chitosan solution and 3.2 ml of glutaraldehyde as a cross linking agent was added into the mixture dropwise. NPs were washed several times with ethanol 70%, dried under vacuum, prepared for chemical characterization and biological assessment. Our data showed that the slow release of DDC-chitosan-NPs resulted in mortality effects on Schistosomal worms in vitro when applied with small dose for longer time. These data compared to those of prazoquantil (PZQ) as a reference drug showed that administration of DDC loaded with chitosan-NPs was more effective on the mortality of Schistosoma worms. In conclusion, DDC-Chitosan-NPs have lethal effects on S. mansoni worms in vitro by a unique mode of action in a way of concentration and exposure time dependent. Also we recommend that the development of DDC as a local, cheaper and more effective agent against S. mansoni worms.
Keywords
Metformin; Hamsters; Pancreatic cancer; N-Nitrosobis (2-oxopropyl) amine
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