ESTABLISHMENT OF CALLUS AND CELL SUSPENSION CULTURES OF CASSIA BICAPSULARIS L. | ||||
| Bulletin of Pharmaceutical Sciences Assiut University | ||||
| Article 3, Volume 36, Issue 1, June 2013, Page 23-30 PDF (159.63 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bfsa.2013.63196 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Iman A. M. Abdel-Rahman* 1, 2; Afaf M. Abdel-Baky1; Ezz El-Din K. Desoky1; Amany S. Ahmed1; Ludger Beerhues2 | ||||
| 1Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt | ||||
| 2Institute of Pharmaceutical Biology, Technische Universität Braunschweig, Mendelssohnstrasse 1, 38106 Braunschweig, Germany | ||||
| Abstract | ||||
| Callus cultures were initiated from leaf of Cassia bicapsularis L. on solid Murashige & Skoog (MS) basal medium supplemented with different growth regulators. Excellent growth of callus was obtained in medium supplemented with 1 mg/l 2,4-dichlorophenoxyacetic acid (2,4- D) and grown in the dark. The obtained callus was subcultured every 4 weeks in the dark at 25°C. The Callus was compact, yellowish brown in colour and used for establishment of cell suspension cultures. Maximum growth of suspension cultures was achieved in medium supplemented with 1 mg/l 2,4-D and 0.1 mg/l kinetin. The growth rate of cells was initially slow but as the cultures proceeded, the growth increased significantly over a period of 22 days then the growth of cells was stable for 35 days. | ||||
|
Statistics Article View: 166 PDF Download: 215 |
||||
