ESTABLISHMENT OF CALLUS AND CELL SUSPENSION CULTURES OF CASSIA BICAPSULARIS L. | ||
| Bulletin of Pharmaceutical Sciences Assiut University | ||
| Article 3, Volume 36, Issue 1, June 2013, Pages 23-30 PDF (159.63 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bfsa.2013.63196 | ||
| Authors | ||
| Iman A. M. Abdel-Rahman* 1, 2; Afaf M. Abdel-Baky1; Ezz El-Din K. Desoky1; Amany S. Ahmed1; Ludger Beerhues2 | ||
| 1Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt | ||
| 2Institute of Pharmaceutical Biology, Technische Universität Braunschweig, Mendelssohnstrasse 1, 38106 Braunschweig, Germany | ||
| Abstract | ||
| Callus cultures were initiated from leaf of Cassia bicapsularis L. on solid Murashige & Skoog (MS) basal medium supplemented with different growth regulators. Excellent growth of callus was obtained in medium supplemented with 1 mg/l 2,4-dichlorophenoxyacetic acid (2,4- D) and grown in the dark. The obtained callus was subcultured every 4 weeks in the dark at 25°C. The Callus was compact, yellowish brown in colour and used for establishment of cell suspension cultures. Maximum growth of suspension cultures was achieved in medium supplemented with 1 mg/l 2,4-D and 0.1 mg/l kinetin. The growth rate of cells was initially slow but as the cultures proceeded, the growth increased significantly over a period of 22 days then the growth of cells was stable for 35 days. | ||
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