MICROBIOLOGICAL ASSAY OF COLISTIN SULFATE ANTIBIOTIC IN PHARMACEUTICAL FORMULATIONS | ||||
| Bulletin of Pharmaceutical Sciences Assiut University | ||||
| Article 1, Volume 36, Issue 2, December 2013, Page 93-103 PDF (1.46 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bfsa.2013.63200 | ||||
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| Authors | ||||
| Omnia H. Albadawy1; Amany M. Nafee1; Momen Thabet* 2; Mostafa S. El-Rehewy1; Ahmed S. Ahmed1 | ||||
| 1Department of Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut 71526, Egypt | ||||
| 2CID Company, Assiut, Egypt | ||||
| Abstract | ||||
| A simple, sensitive and specific agar cup diffusion bioassay for the antibacterial Colistin sulfate was developed. Using a strain of Escherichia coli ATCC 8739 as the test organism, Colistin sulfate at concentrations ranging from 100 to 1600 μg/ml could be measured in pharmaceuticals. A prospective validation of the method showed that the method was linear (r2= 0.999), precise (RSD< 2.8%) and accurate (percent recovery ranges between 98-102%). The method shows that results confirm its precision, not differing significantly from the other method described in the literature. We conclude that microbiological assay is satisfactory using Escherichia coli ATCC 8739 for quantitation of in-vitro antibacterial activity of Colistin sulfate. | ||||
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