CYTOTOXICITY OF ACULEATISIDE-A TO HEPATOCELLULAR CARCINOMA CELLS DETERMINED BY MTT BIOASSAY | ||
| Bulletin of Pharmaceutical Sciences Assiut University | ||
| Article 8, Volume 34, Issue 2, 2011, Pages 121-129 PDF (376.61 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bfsa.2011.63257 | ||
| Authors | ||
| Alaa Mohamed Nafady* 1; Omar Mahmoud Mohamed Mohafez2 | ||
| 1Department of Pharmacognosy, Faculty of Pharmacy, Al-Azhar University, Assiut 71524, Egypt | ||
| 2Department of Biochemistry, Faculty of Pharmacy, Al-Azhar University, Assiut 71524, Egypt | ||
| Abstract | ||
| From the methanolic extract of fresh fruits of Solanum reflexum Schrank, a major compound was isolated and identified as; aculeatiside-A 1. The structure of the isolated compound was elucidated on the basis of 1H and 13C and DEPT-13C-NMR; in addition to comparison with reported data. The cytotoxic effect of aculeatiside-A on hepatocellular carcinoma cells and its mechanism was investigated. It was accomplished that aculeatiside-A inhibit the growth of HepG-2 cells significantly by induction of apoptosis. Marked morphological changes of apoptosis were observed clearly. The results for the first time demonstrate that aculeatiside-A has significant anti-proliferation effect by induction of apoptosis on hepatocellular carcinoma cells in-vitro, down regulation of Survivin and Bcl-2 expressions as well as up regulation of Bax and P53 expression may be one of the important apoptotic inducing mechanisms. | ||
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