Antitumor activity of Neem leaf Extract and Nimbolide on Ehrlich Ascites Carcinoma Cells in Mice
Zahran Mohamed, F., Saad Mohamed, A., Mohamed Abd El-Galil, F., Gamal Haikel, N. (2015). Antitumor activity of Neem leaf Extract and Nimbolide on Ehrlich Ascites Carcinoma Cells in Mice. EKB Journal Management System, 11(1), 27-41. doi: 10.21608/blj.2015.63390
Faten Zahran Mohamed; Amr Saad Mohamed; Fathy Mohamed Abd El-Galil; Noha Gamal Haikel. "Antitumor activity of Neem leaf Extract and Nimbolide on Ehrlich Ascites Carcinoma Cells in Mice". EKB Journal Management System, 11, 1, 2015, 27-41. doi: 10.21608/blj.2015.63390
Zahran Mohamed, F., Saad Mohamed, A., Mohamed Abd El-Galil, F., Gamal Haikel, N. (2015). 'Antitumor activity of Neem leaf Extract and Nimbolide on Ehrlich Ascites Carcinoma Cells in Mice', EKB Journal Management System, 11(1), pp. 27-41. doi: 10.21608/blj.2015.63390
Zahran Mohamed, F., Saad Mohamed, A., Mohamed Abd El-Galil, F., Gamal Haikel, N. Antitumor activity of Neem leaf Extract and Nimbolide on Ehrlich Ascites Carcinoma Cells in Mice. EKB Journal Management System, 2015; 11(1): 27-41. doi: 10.21608/blj.2015.63390

Antitumor activity of Neem leaf Extract and Nimbolide on Ehrlich Ascites Carcinoma Cells in Mice

Biochemistry Letters
Article 2, Volume 11, Issue 1, 2015, Pages 27-41    PDF (1.12 M)
Document Type: Original Article
DOI: 10.21608/blj.2015.63390
Authors
Faten Zahran Mohamed1; Amr Saad Mohamed2; Fathy Mohamed Abd El-Galil2; Noha Gamal Haikel3
1Chemistry department, Biochemistry Division, Faculty of science, Zagazig university, Cairo, Egypt
2Chemistry department, Faculty of science, Cairo university, Cairo, Egypt
3Chemistry department, Faculty of science, Cairo university, Cairo, EgyptNoha Gamal Haikel
Abstract
 




 Background: Azadirachta indica (Neem) has been used traditionally for many centuries. Some impressive therapeutic qualities have been discovered. Aim: Our study aims to investigate in vivo antitumor, and antioxidant activities of Ethanolic Neem Leaf Extract (ENLE) and its fraction Nimbolide, a limonoid present in leaves and flowers of the neem tree (Azadirachta indica). Also, to study the side effects of Ethanolic Neem leaf Extract and Nimbolide fraction on different organs (Liver/ Kidney). Materials & Methods: We assessed the effect of nimbolide and Ethanolic Neem Leaves Extract (ENLE) on life span prolongation and on the levels of malondialdehyde (MDA), nitric oxide (NO), Catalase, glutathione peroxidase (GPx), Caspase-3, and Cytochrome c. Also, our study estimated their effect on Alanine Aminotransferase (ALT), Aspartate Aminotransferase (AST), Total Protein (T.P), Albumin (Alb), bilirubin, urea and creatinine. Results: Ethanolic neem leaves extract and nimbolide showed great increase in life span. Also, they showed a significant decrease in malondialdehyde and nitric oxide and an increase in catalase, glutathione peroxidase, caspase-3 activities, and cytochrome c concentration. Hence, it may be possible that Nimbolide and ENLE decrease lipid peroxidation level due to their antioxidant effect and enhance apoptosis process. Neem leaves extract and nimbolide showed no side effects on liver and kidney. Also, they showed a significant protection for both liver and kidney histopathologically. 
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