Establishment of Radioimmunoassay system for Prostate Specific antigen as a diagnostic tool for prostatic diseases
Elbayoumy, A., Keshta, A., Salam, K., Ebeid, N., Elsheikh, H., Bayoumy, B. (2015). Establishment of Radioimmunoassay system for Prostate Specific antigen as a diagnostic tool for prostatic diseases. EKB Journal Management System, 11(1), 70-79. doi: 10.21608/blj.2015.63396
Ahmed S. Elbayoumy; Akaber T. Keshta; Khaled M. Salam; Nahed H. Ebeid; Hatem M. Elsheikh; Bashir S. Bayoumy. "Establishment of Radioimmunoassay system for Prostate Specific antigen as a diagnostic tool for prostatic diseases". EKB Journal Management System, 11, 1, 2015, 70-79. doi: 10.21608/blj.2015.63396
Elbayoumy, A., Keshta, A., Salam, K., Ebeid, N., Elsheikh, H., Bayoumy, B. (2015). 'Establishment of Radioimmunoassay system for Prostate Specific antigen as a diagnostic tool for prostatic diseases', EKB Journal Management System, 11(1), pp. 70-79. doi: 10.21608/blj.2015.63396
Elbayoumy, A., Keshta, A., Salam, K., Ebeid, N., Elsheikh, H., Bayoumy, B. Establishment of Radioimmunoassay system for Prostate Specific antigen as a diagnostic tool for prostatic diseases. EKB Journal Management System, 2015; 11(1): 70-79. doi: 10.21608/blj.2015.63396

Establishment of Radioimmunoassay system for Prostate Specific antigen as a diagnostic tool for prostatic diseases

Biochemistry Letters
Article 6, Volume 11, Issue 1, 2015, Pages 70-79    PDF (690.11 K)
Document Type: Original Article
DOI: 10.21608/blj.2015.63396
Authors
Ahmed S. Elbayoumy1; Akaber T. Keshta2; Khaled M. Salam1; Nahed H. Ebeid1; Hatem M. Elsheikh1; Bashir S. Bayoumy2
11Department of labeled compounds , Hot labs Centre, Atomic Energy Authority, Egypt.
22Chemistry Department, Faculty of Science, Zagazig University, Egypt.
Abstract
 




 Background: Prostate specific antigen (PSA), a glycoprotein secreted exclusively from epithelial cell lining the prostate gland. The level of PSA in human serum may play a critical role in diagnosis of prostatic diseases. Objectives: This study was carried out to set up Radioimmunoassay system for PSA estimation in human serum as a diagnostic tool for Prostatic diseases. Methods: locally produced highly purified PSA was used for preparation of radioimmunoassay components; PSA Polyclonal antibody, radioiodinated PSA Tracer and PSA standards. Optimization and Formulation have been achieved, then validity of this technique confirmed by measuring the quality control parameters; Sensitivity, accuracy, crossreactivity, intra and inter assay precision and method comparison. Results: we found that; Optimized and Characterized Radioimmunoassay system is the most Sensitive, specific, precise and accurate tool for estimation of prostate specific antigen level in human serum. Conclusion: Estimation of PSA with locally produced radioimmunoassay system can be used as a diagnostic tool for diagnosis of prostatic diseases. 
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