Potential Association of Poly(ADP-ribose) Polymerase-1 (PARP-1) with CD133 and G2/M as Independent Predictors in Colorectal Cancer Development.
El-Emshaty, H., Abdelfatah, A., Ismail, H., El-Taweel, F., El-Hemaly, M. (2019). Potential Association of Poly(ADP-ribose) Polymerase-1 (PARP-1) with CD133 and G2/M as Independent Predictors in Colorectal Cancer Development.. EKB Journal Management System, 15(1), 156-166. doi: 10.21608/blj.2019.63832
Hoda M. El-Emshaty; Amina A. Abdelfatah; Hisham Ismail; Fathy M El-Taweel; Mohamed M. El-Hemaly. "Potential Association of Poly(ADP-ribose) Polymerase-1 (PARP-1) with CD133 and G2/M as Independent Predictors in Colorectal Cancer Development.". EKB Journal Management System, 15, 1, 2019, 156-166. doi: 10.21608/blj.2019.63832
El-Emshaty, H., Abdelfatah, A., Ismail, H., El-Taweel, F., El-Hemaly, M. (2019). 'Potential Association of Poly(ADP-ribose) Polymerase-1 (PARP-1) with CD133 and G2/M as Independent Predictors in Colorectal Cancer Development.', EKB Journal Management System, 15(1), pp. 156-166. doi: 10.21608/blj.2019.63832
El-Emshaty, H., Abdelfatah, A., Ismail, H., El-Taweel, F., El-Hemaly, M. Potential Association of Poly(ADP-ribose) Polymerase-1 (PARP-1) with CD133 and G2/M as Independent Predictors in Colorectal Cancer Development.. EKB Journal Management System, 2019; 15(1): 156-166. doi: 10.21608/blj.2019.63832

Potential Association of Poly(ADP-ribose) Polymerase-1 (PARP-1) with CD133 and G2/M as Independent Predictors in Colorectal Cancer Development.

Biochemistry Letters
Article 12, Volume 15, Issue 1, 2019, Page 156-166  XML PDF (383.2 K)
DOI: 10.21608/blj.2019.63832
View on SCiNiTO View on SCiNiTO
Authors
Hoda M. El-Emshaty1; Amina A. Abdelfatah2; Hisham Ismail3; Fathy M El-Taweel2; Mohamed M. El-Hemaly1
1Gastroenterology Surgical Center, Faculty of Medicine, Mansoura University, Egypt
2Biochemistry Department, Faculty of Science, Damietta University, New Damietta, Egypt
3Biochemistry Division, Faculty of Science, Minia University, Minia, Egypt
Abstract
Objective: PARP inhibitor therapy was intensively investigated in colorectal cancer (CRC). Therefore, our aim was to investigate the potential association of DNA repair protein PARP-1 with cancer stem cell CD133 and DNA cell cycle abnormalities in colorectal cancer patients and ulcerative colitis (UC) as a diagnostic tool for differential diagnosis, evaluation of tumor progression and prediction of patient's disease outcome to benefit in therapeutic response.
Materials and Methods: Thirty seven (20 colorectal cancer and 17 Ulcerative colitis) patients and ten tissue specimens of normal colon mucosa used as non-disease control group. Tissue specimens from all individuals were collected at surgery and examined for PARP-1, CD133 and DNA Cell cycle by flow cytometry.
Results: CD133 and PARP-1 were gradually increased from UC to CRC (p<0.0001) in CD133 and PARP-1 (p=0.02). DNA cell cycle abnormalities showed significant difference between CRC and UC groups only in G2/M (p<0.0001). CRC showed higher expression of CD133 in Stage III compared to stage I (p=0.01) but the difference in tumor site was recorded between transverse colon and rectum in S phase (p=0.04) and G0/1 (p=0.016) and between transverse and Lt Colon in G0/1 (p=0.016). Multiple regressions for PARP-1, CD133 and G2/M showed higher prediction for CRC progression.
Conclusion: PARP-1, CD133 and G2/M could be considered as additional biomarkers to increase the diagnostic potential in CRC patients, in predicting tumor development and monitoring therapeutic response.   
Keywords
CD133; PARP-1; DNA cell cycle; Colorectal cancer (CRC); Ulcerative colitis (UC); Flow cytometry
Statistics
Article View: 360
PDF Download: 289