PRODUCTION OF AN EXTRACELLULAR HALOPHILIC AMYLASE FROM THE EXTREMELY HALOPHILIC ARCHAEON NATRIALBA AEGYPTIACA STRAIN 40T | ||||
| Al-Azhar Bulletin of Science | ||||
| Article 18, Volume 2013, Issue 12, December 2013, Page 93-108 PDF (279.94 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/absb.2013.6422 | ||||
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| Authors | ||||
| NOURA HAGAGGI1; FRANCIS F. HEZAYEN1; USAMA M. ABDULRAOUF* 2 | ||||
| 1Botany Department, Faculty of Science, Aswan University, Aswan, Egypt | ||||
| 2Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Assiut, Egypt | ||||
| Abstract | ||||
| The extremely halophilic archaeon, Natrialba aegyptiaca strain 40T, which was isolated from a salty soil close to Aswan, Egypt is able to produce a halophilic extracellular, raw starch-digesting amylase. Optimization of medium components and culture conditions to enhance amylase production was investigated. Maximum production of this amylase was achieved in a medium contained (%, w/v): NaCl, 15; KCl, 1.5; MgSO4.7H2O, 0.1 and soluble starch, 0.05 at a pH range between 6-8 and a temperature range between 45-47 oC after incubation period of 144 hr. under static conditions and 48 hr. with shaking at 150 rpm. The enzyme could efficiently hydrolyze raw starches from different plant sources. | ||||
| Keywords | ||||
| halophilic; Natrialba aegyptiaca; amylase; production; Optimization; raw starches | ||||
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