Detection of Antibiotic Resistant Genes in Salmonella Isolated From Poultry
Eid, H., Nasef, S., Hassonna, N. (2015). Detection of Antibiotic Resistant Genes in Salmonella Isolated From Poultry. EKB Journal Management System, 20(2), 175-189. doi: 10.21608/scvmj.2015.64626
Hamza Eid; Soad Nasef; Nosaiba Hassonna. "Detection of Antibiotic Resistant Genes in Salmonella Isolated From Poultry". EKB Journal Management System, 20, 2, 2015, 175-189. doi: 10.21608/scvmj.2015.64626
Eid, H., Nasef, S., Hassonna, N. (2015). 'Detection of Antibiotic Resistant Genes in Salmonella Isolated From Poultry', EKB Journal Management System, 20(2), pp. 175-189. doi: 10.21608/scvmj.2015.64626
Eid, H., Nasef, S., Hassonna, N. Detection of Antibiotic Resistant Genes in Salmonella Isolated From Poultry. EKB Journal Management System, 2015; 20(2): 175-189. doi: 10.21608/scvmj.2015.64626

Detection of Antibiotic Resistant Genes in Salmonella Isolated From Poultry

Suez Canal Veterinary Medical Journal. SCVMJ
Article 13, Volume 20, Issue 2, December 2015, Pages 175-189    PDF (1.77 M)
Document Type: Original Article
DOI: 10.21608/scvmj.2015.64626
Authors
Hamza Eid1; Soad Nasef2; Nosaiba Hassonna* 3
1Dept. of Bacteriology, Mycology and Immunology, Fac. of Vet. Medicine, Suez Canal Univ., Egypt
2Dept. of Bacteriology,Mycology and Immunology, Fac. of Vet. Medicine, Suez Canal Univ., Egypt
3Animal Health Research Institute , Dokki, Egypt
Abstract
Three hundred samples of infected and freshly dead chickens from different farms gathered in Hehia city El-sharkia  for  bacteriological examination  to  detect the presence of Salmonella, 23 Salmonella isolates were detected from 300 samples.Serological results  detected Salmonella enteritidis  with percentage 26.1 % , followed by Salmonella typhimurium  with percentage 17.4 %  and Salmonella kentucky 8.7% . All Salmonella isolates were tested for their susceptibility to the following antimicrobial agents: gentamicin(CN), ,ciprofloxacin (CIP), amoxicillin- clavulanic acid (AMC), , doxycyclin (DO), chloramphenicol (C), erythromycin (E), sulfamethoxazole -trimethoprim (SXT)  High rate of susceptibility was the most common finding obtained against ciprofloxacin (75%)   as shown in table (24). Also, absolute resistance was obtained among Salmonella isolates against erythromycin (100%) and amoxicillin clavulanic acid (100%),  and sulfamethoxazole -trimethoprim (25%).In addition, 33.3% of  isolates were resistant to chloramphenicol, and colistin sulfate. All isolates were resistant to at least three antibiotics and multidrug resistance was seen .PCR detected 6 types of antibiotic resistant genes (aadB gene, qnrS gene ,sul1 gene , floR gene ,dfrA gene and blaTEM  gene) in percentage  91.7% ,83.3% ,66.7%, 75%,33.3% and 83.3% respectively .
Keywords
Salmonella; Antibiotic resistance; genes; Poultry
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