Micropropagation of Four Coffee Cultivars (Coffea arabica L.) from Yemen through Shoot Tip Culture | ||
| Hortscience Journal of Suez Canal University | ||
| Article 4, Volume 4, Issue 1, January 2015, Pages 25-31 PDF (1.69 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/hjsc.2015.6472 | ||
| Authors | ||
| Ameen AL-Azab1; Sanad Habib1; Mohammed Hussein2; Fadia El-Sherif1 | ||
| 1Horticulture Dept., Faculty of Agriculture, Suez Canal University, 41522, Ismailia, Egypt | ||
| 2Botany Dept., Faculty of Agriculture, Suez Canal University, 41522, Ismailia, Egypt. | ||
| Abstract | ||
| The aim of this study is to optimize in vitro multiplication of Coffea arabica cvs 'Benan',' Burai',' Odayni' and 'Odayni- Bayat'. Seeds germinated on modified quarter MS medium with 1.0 g l−1 activated charcoal gave the highest germination percentage of the four cultivars under study. BA at 8.0 mgl-1 was the most effective concentration with an average of 14 shoots per explant, resulting in better and morphologically superior microshoots in 'Benan' cv. However 'Burai' ,'Odayni' and 'Odayni- Bayat' cultivars, showed the highest number of shoots when they were grown on MS medium supplemented with BA at 4.0, 2.0 and 6.0 mgl-1 respectively. Root formation was 100% in response to the application of IAA, IBA and /or NAA to the culture media for the four cultivars. Half MS supplemented with NAA at 2.0, 1.0 and 3.0 mgl-1 was found to be a suitable medium for root induction in excised micro-shoots, of 'Odayni', 'Burai' and 'Odayni-Bayat' cultivars, respectively. The plantlets were successfully acclimatized in the greenhouse; consequently the survived plants reached 100%. | ||
| Keywords | ||
| in Vitro; benzyladenine; Coffea arabica. 'Benan'; 'Burai'; 'Odayni' and Odayni-Bayat; micropropagation | ||
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