Preliminary Detection of Rickettsiae Using PCR Targeting OmpA Gene Among Dogs and Horses in Cairo, Egypt
Abdullah, H., El-Molla, A., Saleb, F., Ghazy, A., Allam, N., Abdelshafy, S. (2019). Preliminary Detection of Rickettsiae Using PCR Targeting OmpA Gene Among Dogs and Horses in Cairo, Egypt. EKB Journal Management System, 50(The 8th lnternational Conference of Veterinary Research Division (NRC) Cairo, Egypt , 3rd-5th December, 2019), 1-8. doi: 10.21608/ejvs.2019.19553.1127
Hend H.A.M. Abdullah; Amal El-Molla; Fayez A. Saleb; Alaa A Ghazy; Nesreen A.T. Allam; Sobhy Abdelshafy. "Preliminary Detection of Rickettsiae Using PCR Targeting OmpA Gene Among Dogs and Horses in Cairo, Egypt". EKB Journal Management System, 50, The 8th lnternational Conference of Veterinary Research Division (NRC) Cairo, Egypt , 3rd-5th December, 2019, 2019, 1-8. doi: 10.21608/ejvs.2019.19553.1127
Abdullah, H., El-Molla, A., Saleb, F., Ghazy, A., Allam, N., Abdelshafy, S. (2019). 'Preliminary Detection of Rickettsiae Using PCR Targeting OmpA Gene Among Dogs and Horses in Cairo, Egypt', EKB Journal Management System, 50(The 8th lnternational Conference of Veterinary Research Division (NRC) Cairo, Egypt , 3rd-5th December, 2019), pp. 1-8. doi: 10.21608/ejvs.2019.19553.1127
Abdullah, H., El-Molla, A., Saleb, F., Ghazy, A., Allam, N., Abdelshafy, S. Preliminary Detection of Rickettsiae Using PCR Targeting OmpA Gene Among Dogs and Horses in Cairo, Egypt. EKB Journal Management System, 2019; 50(The 8th lnternational Conference of Veterinary Research Division (NRC) Cairo, Egypt , 3rd-5th December, 2019): 1-8. doi: 10.21608/ejvs.2019.19553.1127

Preliminary Detection of Rickettsiae Using PCR Targeting OmpA Gene Among Dogs and Horses in Cairo, Egypt

Egyptian Journal of Veterinary Sciences
Article 1, Volume 50, The 8th lnternational Conference of Veterinary Research Division (NRC) Cairo, Egypt , 3rd-5th December, 2019, December 2019, Page 1-8  XML PDF (1.19 MB)
Document Type: Original Article
DOI: 10.21608/ejvs.2019.19553.1127
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Authors
Hend H.A.M. Abdullahorcid 1; Amal El-Molla2; Fayez A. Saleb3; Alaa A Ghazyorcid 4; Nesreen A.T. Allamorcid 4; Sobhy Abdelshafy email orcid 5
1National Research Centre, Dokki, Giza, Egypt
2Faculty of Veterinary Medicine, Cairo University
3Faculty of Veterinary Medicine Cairo University
4National Research Centre, Dokki, Giza, Egypt.
5Department of Parasitology and Animal Diseases, Veterinary Research Division, National Research Centre, Dokki, Giza, Egypt.
Abstract
Background: Rickettsioses are intracellular bacterial infectious diseases causing high morbidity and sometime high mortality with public health importance.
Aim: The aim of present study was to detect rickettsiae in dogs and horses in Cairo, Egypt by using Polymerase Chain Reaction (PCR) targeting OmpA gene.
Methods: A total number of 200 blood samples (100 from dogs and 100 from horses) were collected from Cairo province, Egypt. All blood samples were investigated by PCR using OmpA gene to detect the rickettsial infection. Furthermore, hematological examination for all blood samples was performed.
Results: PCR detection of rickettsiae using OmpA gene amplification revealed that the infection rate was 18 % and 72 % in dogs and horses, respectively. Regarding hematological changes, hypochromic anemia, significant increase in neutrophils, lymphopenia and monocytopenia were recorded in rickettsiae positive dogs. Meanwhile, thrombocytopenia, leucopenia and monocytosis were recorded in rickettsiae positive horses.
Conclusion: The detection of rickettsiae in dogs and horses indicated that dogs and horses play a critical role as sentinels in an epidemiological approach of rickettsiae in Cairo, Egypt.
Keywords
Rickettsiae; dogs; Horses; PCR; OmpA gene
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