SIMULTANEOUS HPLC-UV QUANTIFICATION OF DILTIAZEM AND N-DEMETHYLDILTIAZEM IN HUMAN PLASMA | ||||
| Bulletin of Pharmaceutical Sciences Assiut University | ||||
| Article 1, Volume 29, Issue 1, 2006, Page 1-8 PDF (256.85 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bfsa.2006.64822 | ||||
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| Authors | ||||
| S. S. Hasan* 1; Nisar ur Rahman2; Khalid Hussain1; S. Atif Raza1 | ||||
| 1University College of Pharmacy, Punjab University, Allama Iqbal Campus, Lahore-54000, Pakistan | ||||
| 2Department of Pharmacy, Islamia University Bahawalpur, Pakistan | ||||
| Abstract | ||||
| A simple and sensitive reversed phase high-performance liquid chromatographic method was developed for simultaneous quantification of diltiazem HCl and its major metabolite Ndemethyldiltiazem in human plasma. The method involves one step solvent extraction of diltiazem, N-demethyldiltiazem and the internal standard, verapamil with n-hexane and diethyl ether (50:50 v/v). The mobile phase comprised 0.1 M ammonium dihydrogen phosphate-acetonitrile (62:38 v/v) and triethylamine (0.08%) was added before the pH was adjusted to 5.9 with 85% phosphoric acid. Analysis was run at a flow rate of 1.0 ml/min at a detection wavelength of 238 nm. The completion time for assay was not more than 10 minutes and lower limit of quantification was 5 ng/ml. The calibration curve for diltiazem and its metabolite was linear over a concentration range of 5-200 ng/ml and average recovery was about 90%. The coefficient of variation and percent error values of the assay method within and between days were all less than 10%. | ||||
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