Serological and Molecular Studies on Multi-drug Resistant Salmonella Isolated from Captive Budgerigars (Melopsittacus undulatus) | ||
| Suez Canal Veterinary Medical Journal. SCVMJ | ||
| Article 10, Volume 20, Issue 1, June 2015, Pages 135-145 PDF (1.28 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/scvmj.2015.65028 | ||
| Authors | ||
| Enas Saad* 1; Atef Kamel1; Ahmed Khafagy2; Mohamed Gamal Eldein1 | ||
| 1Wildlife Management and Zoo Medicine Dept. Faculty of Veterinary Medicine. Suez Canal University. | ||
| 2Microbiology Dept. , Faculty of Veterinary Medicine. Suez Canal University. | ||
| Abstract | ||
| The present work was carried out for serological and molecular screening of virulence genes associated with Salmonella in captive budgerigars. A total of 805 apparently healthy birds were collected from different sources, and subjected to clinical and bacteriological examination. Salmonella species were isolated at rate of (4.97%) with recognition of 4 different serovars in which Salmonella Paratyphi A was the most common isolated serotype. All isolates were highly sensitive to Ciprofloxacin, Enrofloxacin and Norfloxacin. Multiplex-PCR using (invA, spvC and stn) was devised to confirm the isolates and predict their virulence. (invA;284bp) was detected in all Salmonella isolates with (100%), while (stn;617bp)and (spvC; 392bp) were detected in some Salmonella isolates. | ||
| Keywords | ||
| Multi-drug Resistant; Salmonella; Captive Budgerigars | ||
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