ANTICANCER GLUTAMINASE PRODUCTION AND OPTIMIZATION USING HALOTOLERANT ASPERGILLUS FLAVUS CZCU-9, F1H
Sidkey, N., Sidkey, N., Abo Elsoud, M., Elnemr, W., Elhateir, M. (2019). ANTICANCER GLUTAMINASE PRODUCTION AND OPTIMIZATION USING HALOTOLERANT ASPERGILLUS FLAVUS CZCU-9, F1H. EKB Journal Management System, 2019(6), 1-9. doi: 10.21608/absb.2019.66073
Nagwa Sidkey; Nagwa M. Sidkey; Mostafa M. Abo Elsoud; Wessam Elnemr; Mai M. Elhateir. "ANTICANCER GLUTAMINASE PRODUCTION AND OPTIMIZATION USING HALOTOLERANT ASPERGILLUS FLAVUS CZCU-9, F1H". EKB Journal Management System, 2019, 6, 2019, 1-9. doi: 10.21608/absb.2019.66073
Sidkey, N., Sidkey, N., Abo Elsoud, M., Elnemr, W., Elhateir, M. (2019). 'ANTICANCER GLUTAMINASE PRODUCTION AND OPTIMIZATION USING HALOTOLERANT ASPERGILLUS FLAVUS CZCU-9, F1H', EKB Journal Management System, 2019(6), pp. 1-9. doi: 10.21608/absb.2019.66073
Sidkey, N., Sidkey, N., Abo Elsoud, M., Elnemr, W., Elhateir, M. ANTICANCER GLUTAMINASE PRODUCTION AND OPTIMIZATION USING HALOTOLERANT ASPERGILLUS FLAVUS CZCU-9, F1H. EKB Journal Management System, 2019; 2019(6): 1-9. doi: 10.21608/absb.2019.66073

ANTICANCER GLUTAMINASE PRODUCTION AND OPTIMIZATION USING HALOTOLERANT ASPERGILLUS FLAVUS CZCU-9, F1H

Al-Azhar Bulletin of Science
Article 1, Volume 2019, Issue 6, June 2019, Pages 1-9    PDF (477.99 K)
Document Type: Original Article
DOI: 10.21608/absb.2019.66073
Authors
Nagwa Sidkey1; Nagwa M. Sidkey1; Mostafa M. Abo Elsoud2; Wessam Elnemr1; Mai M. Elhateir* 1
1Department of Botany and Microbiology, Faculty of Science, Al - Azhar U n iversity (Girls Branch), Youssif Abbas St., Nasr City, Cairo, Egypt
2National Research Center (NRC), Dokki, Cairo, Egypt
Abstract
Isolation of halotolerant glutaminase producing microorganisms was carried out using medium containing different concentrations of NaCl viz. 2.9, 3.5 and 4 %. Only four fungal isolates (F1H, F2H, F3H and F4H) were able to grow at all concentrations has been selected and purified. Production of glutaminase was carried out in broth medium containing 4% NaCl. Quantitaive assay for glutaminase led to 6.43, 5.72, 6.12 and 5.86 U/ml for each isolate extracellularly, respectively. In case of intracellular production assay led to 5.2, 5.46, 5.18 and 5.77 U/ml , respectively. The isolate F1H has showed the highest productivity extracellularly and stability on subculturing so, it was chosen and selected for furthur investigations. The optimized conditions for glutaminase production by isolate F1H was found to be 6 days at 35oC and pH 7 under static and dark incbation conditions with inoculum size of 4.38x106 spores/ ml. The optimization led to production of 7.66 U/ml of enzyme with great stability at 4% salt concentration which is a good sign for probability that the enzyme could have unique properties which can be confirmed through more investigatios.The isolate was identified genetically and has shown 100% similarity to Aspergillus flavus CZCU-9 and it was given the name Aspergillus flavus CZCU-9, F1H.
Keywords
Halotolerant; glutaminase; Anticancer; extracellular; intracellular; Optimization
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