Role of Alpha Smooth Muscle Actin in Oral Squamous Cell Carcinoma Progression | ||
| Egyptian Dental Journal | ||
| Article 16, Volume 65, Issue 3 - July (Oral Medicine, X-Ray, Oral Biology & Oral Pathology), July 2019, Pages 2387-2396 PDF (3.34 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/edj.2019.72277 | ||
| Authors | ||
| H. El-Kammar1; Nermeen S. Afifi2; D. AbdulKhalik3 | ||
| 1Lecturer of Oral Pathology, Faculty of Oral and Dental Medicine, Future University in Egypt | ||
| 2Lecturer of Oral Pathology, Faculty of Dentistry, Ain-Shams University | ||
| 3Assisstant Professor of Oral Biology, Faculty of Dentistry, Ain-shams University | ||
| Abstract | ||
| Squamous cell carcinoma (SCC) is the most common type of oral cancer. Malignant epithelal cells undergo cytological changes by a process referd to as epithelial mesenchymal transition (EMT). The cancer associated fibroblasts (CAFs) in the tumor micro-environment are now the focus of intense research and are believed to correlate with poor prognosis. They are characterized by alpha smooth muscle actin (α-SMA) expression, which is a myofibroblastic marker. Physiologically myofibroblasts are not as abundant as fibroblasts in the oral cavity, they have limited locations such as blood vessels walls in the oral mucosa. Aim: evaluation of the potential role of α-SMA in the progression of SCC. Materials and methods: qRT-PCR was performed to evaluate α-SMA gene expression in tongue SCC cell line (SCC 25), normal fibroblasts cell line (Wi-38) and in a coculture of both cells. Qualitative and quantitative immunohistochemical analysis of α-SMA expression with clinico-pathological correlations was performed for 24 SCC specimens of different grades. Results: qRT-PCR results showed that there was α-SMA expression in the epithelial cells, and that co-culturing resulted in an overall increase in α-SMA expression. Immunohistochemical evaluation of the specimens revealed that the expression of α-SMA increased with tumor grade and correlated with lymph node involvement. Conclusion: α-SMA can be used as a prognostic marker and a potential target for cancer therapy. | ||
| Keywords | ||
| Squamous cell carcinoma; myofibroblasts; Alpha smooth muscle actin; cancer associated fibroblasts; epithelial mesenchymal transition | ||
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