ANALYSIS OF DNA PLOIDY AND S-PHASE FRACTION IN RELATION TO DEVELOPMENT OF ORAL SQUAMOUS CELL CARCINOMA: AN EXPERIMENTAL STUDY | ||||
| Egyptian Dental Journal | ||||
| Article 22, Volume 65, Issue 3 - July (Oral Medicine, X-Ray, Oral Biology & Oral Pathology), July 2019, Page 2447-2455 PDF (1.14 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/edj.2019.72898 | ||||
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| Authors | ||||
| Ahmed M. Hussein1; Sahar M. El-Sheikh2; Mahmoud A. Attia3; Omneya R. Ramadan4; Enas M. Omar5; Ahmed H. Gaber6 | ||||
| 1Lecturer of Oral and Maxillofacial Pathology Department, Faculty of Oral and Dental Medicine, SVU, Qena, Egypt | ||||
| 2Dean of Faculty of Dentistry, Professor of Oral Pathology, Faculty of Dentistry, Alexandria University, Alexandria, Egypt | ||||
| 3Assistant Professor of Oral Pathology, Department of Oral Pathology, Faculty of Dental Medicine, Al-Azhar University, Assiut Branch, Egypt | ||||
| 4Assistant Professor of Oral Pathology, Faculty of Dentistry, Alexandria University, Alexandria, Egypt | ||||
| 5Lecturer of Oral Pathology, Faculty of Dentistry, Alexandria University, Alexandria, Egypt | ||||
| 6Lecturer of Oral Pathology, Department of Oral Pathology, Faculty of Dental Medicine, Al-Azhar University, Assiut Branch, Egypt | ||||
| Abstract | ||||
| Introduction: Cancer is a serious public health problem in many parts of the world; oral squamous cell carcinoma was the 11th most common malignant neoplasm in human papulation. The hamster cheek pouch carcinogenesis model is a well-known animal system that closely mimics the development of premalignant and malignant lesions in human oral cancer. The expressions of cancerization process in this model using DNA ploidy and S-phase fraction is the point of interest. Aim of the study: This research was carried out to study the analysis of DNA ploidy and S-phase fraction in relation to development of oral squamous cell carcinoma during hamster cheek pouches carcinogenesis process induced by DMBA. Material and methods: A total of 50 young gold Syrian hamsters distributed into groups as follows: 2 normal animals examined for the normal histology of the cheek pouch mucosa and 48 animals divided into; group I, as a control group (n= 12), in which pouches were painted with a heavy mineral oil only; group II, were painted with DMBA mixed in a heavy mineral oil (n=36). The examined animal tissue utilized for flow cytometry analysis to obtain DNA ploidy and S-phase fraction development during carcinogenesis process. Results: The flow cytometric analysis of DNA content demonstrated a significant role in carcinogenesis process in oral squamous cell carcinoma. Moreover, they provided a significant analysis in the proliferation and activity of the cancer cells as measured by the S-phase fraction activity. Conclusions: The combinatorial DNA content analysis involving general ploidy and S-phase fraction have potential for support in the early diagnosis; during the development of oral squamous cell carcinoma. | ||||
| Keywords | ||||
| DNA ploidy; S-phase fraction; Flow cytometry; Squamous cell carcinoma | ||||
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