Characterization and Purification of Alkaline Proteases From Viscera of Silver Carp (Hypophthalmichthys molitrix) Fish | ||||
| Assiut Journal of Agricultural Sciences | ||||
| Article 4, Volume 48, Issue 6, December 2017, Page 40-54 PDF (492.36 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ajas.2017.7572 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| M. B. Atta* 1; Shimaa El-Said Abd El-Hamed2; Samia A. Keshk2 | ||||
| 1Food Science and Technology Department, Faculty of Agriculture, Tanta University | ||||
| 2Food Science and Technology Institute, Agriculture Research Center, Ministry of Agriculture | ||||
| Abstract | ||||
| Proteases from viscera of Silver carp (Hypophthalmichthys molitrix) fish have been extracted and characterized. The alkaline proteases show optimum activity in 0.2M Tris-HCl buffer at pH 8.5 and 45°C using soluble milk casein as substrate. The crude alkaline protease lost about 35% or 51% of its specific activity if it was heated at 50°C or kept at pH 9 for 60 min., respectively. Purification of proteases purified by ammonium sulfate precipitation, gel-filtration using Sephadex G 50 column and ultrafiltration via polyamide membrane (30 KDa) led to increase its specific activity up to 20, 24 and 99 fold, respectively. | ||||
| Keywords | ||||
| Silver carp; alkaline proteases; Characterization; Purification; specific activity | ||||
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