Molecular Characterization of β-lactamase Genes in Antibiotic Resistant Bacteria | ||
| Zagazig Veterinary Journal | ||
| Article 7, Volume 45, Issue 1, March 2017, Pages 55-63 PDF (600.62 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/zvjz.2017.7687 | ||
| Authors | ||
| Adel M. Attia; Mohammed N. Hassan; Shymaa E. Elwan* | ||
| Microbiology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt | ||
| Abstract | ||
| Beta-lactamases (bls) are implicated seriously in resistance to β - lactam antibiotics. β- lactam resistance related to Sulfa hydral variabl- beta lactamases (SHV- bls) were detected in E. coli and Klebsiellae isolated from human and chickens. In this work, resistance profile of the isolates, extended spectrum β-lactamases (ESBLs) activity and SHV genes sequencing were examined. E. coli was isolated from humans, broiler and day-old chicks samples, meanwhile, K. pneumonia was isolated only from human samples (88.8%). Sixty-four (88.8 %) of human isolates contained beta-lactamases (iodometric positive), 28% were ESBLs and 35% contained SHV. In case of day-old chick isolates, 50% contained beta-lactamases (iodometric positive), 5.5% were ESBLs. Out of 45 broiler isolates, 37.7% contained beta-lactmases (iodometric positive), 8.8% were ESBLs and 80% contained SHV. It was found that SHV were of SHV-5 and SHV-12.It could be concluded that, wide spread of ESBLs SHV-5 and SHV-12 in humans and chicken is a major concern in resistance to β -lactam drugs. | ||
| Keywords | ||
| Extended spectrum beta lactamases; Sulfa hydral variable (SHV); E. coli; Klebsiella pneumoniae | ||
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