Evaluation of Antibacterial Effect of Diode laser 980 nm, Triple Antibiotic Past, and Calcium Hydroxide on Enterococcus Faecalis Biofilm
Obeid, M., Nour, M. (2018). Evaluation of Antibacterial Effect of Diode laser 980 nm, Triple Antibiotic Past, and Calcium Hydroxide on Enterococcus Faecalis Biofilm. EKB Journal Management System, 64(Issue 3 - July (Fixed Prosthodontics, Dental Materials, Conservative Dentistry & Endodontics)), 2863-2870. doi: 10.21608/edj.2018.77359
Maram Farouk Obeid; Mona Saad Nour. "Evaluation of Antibacterial Effect of Diode laser 980 nm, Triple Antibiotic Past, and Calcium Hydroxide on Enterococcus Faecalis Biofilm". EKB Journal Management System, 64, Issue 3 - July (Fixed Prosthodontics, Dental Materials, Conservative Dentistry & Endodontics), 2018, 2863-2870. doi: 10.21608/edj.2018.77359
Obeid, M., Nour, M. (2018). 'Evaluation of Antibacterial Effect of Diode laser 980 nm, Triple Antibiotic Past, and Calcium Hydroxide on Enterococcus Faecalis Biofilm', EKB Journal Management System, 64(Issue 3 - July (Fixed Prosthodontics, Dental Materials, Conservative Dentistry & Endodontics)), pp. 2863-2870. doi: 10.21608/edj.2018.77359
Obeid, M., Nour, M. Evaluation of Antibacterial Effect of Diode laser 980 nm, Triple Antibiotic Past, and Calcium Hydroxide on Enterococcus Faecalis Biofilm. EKB Journal Management System, 2018; 64(Issue 3 - July (Fixed Prosthodontics, Dental Materials, Conservative Dentistry & Endodontics)): 2863-2870. doi: 10.21608/edj.2018.77359

Evaluation of Antibacterial Effect of Diode laser 980 nm, Triple Antibiotic Past, and Calcium Hydroxide on Enterococcus Faecalis Biofilm

Egyptian Dental Journal
Article 48, Volume 64, Issue 3 - July (Fixed Prosthodontics, Dental Materials, Conservative Dentistry & Endodontics), July 2018, Pages 2863-2870    PDF (697.32 K)
Document Type: Original Article
DOI: 10.21608/edj.2018.77359
Authors
Maram Farouk Obeid1; Mona Saad Nour2
1Lecturer of Endodontics, Department of Endodontics, Faculty of Dentistry, Ain Shams University.
2Lecturer of Medical Microbiology and Immunology, Department of Medical Microbiology and Immunology Faculty of Medicine- Ain Shams University
Abstract
Aim: The purpose of this study is to evaluate the anti-biofilm capacity of Diode laser 980 nm, triple antibiotic mixture and calcium hydroxide.
Methods: Eighty five single-rooted teeth with mature apices were prepared using Protaper Universal rotary nickel titanium system till size # F4 then contaminated with E.faecalis. Irrigation done using 2.5% sodium hypochlorite followed by 17% EDTA. Samples were divided into 4 groups (n= 20) according to the irrigant activation method. Laser group,TAP group, Ca(OH)2group and the control group. Residual bacteria were plated onto Brain Heart Infusion media and determined as colony-forming units (CFU mL-1). Data were analyzed using one way ANOVA followed by performance of Tukey post hoc tests. Significance was set at p < 0.05.
Results: There was a statistically significant reduction in the mean numbers of colony-forming units among all groups. However, none of the activation methods was able to kill E. faecalis biofilm completely. The Laser group behaved most effectively among all groups.
Conclusion: The adjunctive use of 980nm laser is an effective method for bacterial reduction after chemo-mechanical instrumentation of the root canal.
Keywords
Diode laser; TAP; antibiofilm activity
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