Growth hormone modulates the inflammatory and apoptotic pathways incorporated in fluorouracil-induced oral mucositis in rats | ||||
| Egyptian Dental Journal | ||||
| Article 17, Volume 66, Issue 1 - January (Oral Medicine, X-Ray, Oral Biology & Oral Pathology), January 2020, Page 327-336 PDF (2.83 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/edj.2020.77550 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Rasha H. Al Serwi1; Samar F. Darwish2; Yasmen F. Mahran3 | ||||
| 1Oral Biology Department, Faculty of Dentistry, Al- Mansoura University | ||||
| 2Pharmacology, Toxicology & Biochemistry Department, Faculty of Pharmacy, Badr University in Cairo, Egypt | ||||
| 3Department of Pharmacology & Toxicology, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt | ||||
| Abstract | ||||
| Introduction: Oral mucositis (OM) is a well-known complication of radiotherapy (RT) and chemotherapy (CT) in cancer patients. Although, 5-flourouracil (5-FU) is one of the standard cytotoxic therapies, it is one of the most common causes of OM, which results in delay, dose reduction or treatment discontinuation, thus this intensifies the need for an effective chemoprotective agent. We aimed to investigate the potential chemoprotective effect of growth hormone (GH) on 5-FU-induced OM in rats. Material & Methods: Rats were either exposed to a single 5-FU injection (160 mg/kg ip) and/or treated with GH (1 mg/kg s.c). Oxidative stress, inflammatory and apoptotic markers were assessed. Results: An array of mucosal damage was produced as a result of 5-FU injection, which was evident by histopathology. 5-FU induced a remarkable increase in lipid peroxidation accompanied with a significant depletion of glutathione level. Besides, inflammatory cascades, including NF-κB and IL-6, were elevated significantly. Furthermore, 5-FU stimulated cell death through the significant increase of caspase-3. Interestingly, Pre-treatment with GH markedly ameliorated the deleterious effects of 5-FU through counteracting the oxidative stress and inflammation-mediated apoptosis. In addition, GH rescued the oral mucosal histology following chemotherapy. Conclusions: Our study is the first to demonstrate evidences for the effective chemoprotection provided by GH against 5-FU induced OM along with the underlying mechanisms, which may offer a promising adjuvant therapy for cancer patients. | ||||
| Keywords | ||||
| Growth hormone; Chemotherapy; 5- Fluorouracil; Oral mucositis; Inflammation | ||||
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