Phenotypic and genotypic characterization of S. aureus isolated from clinical and subclinical bovine mastitis | ||
| Suez Canal Veterinary Medical Journal. SCVMJ | ||
| Article 12, Volume 18, Issue 1, June 2013, Pages 139-147 PDF (330.25 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/scvmj.2013.78287 | ||
| Authors | ||
| Mohamed Enany1; Soha Younes2; Abd Elazeem AL gammal1; Mohamed Salem3; Hoda El Dieb* 1 | ||
| 1Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Suez Canal University | ||
| 2Department of Clinical pathology, Faculty of Medicine, Suez Canal University | ||
| 3Department of Animal Medicine, Faculty of Veterinary Medicine, Suez Canal University | ||
| Abstract | ||
| In order to determine the prevalence of S. aureus in clinical and subclinical bovine mastitis as well as the relationship between the presence of protein A gene and the udder inflammatory response . A total of 400 quarter milk samples were collected from 100 lactating Friesian cows at Ismailia Governorate . The percentage of clinical mastitis was ( 19.5%.), while the percentage of subclinical mastitis according to C.M.T was ( 42.85%) . The percentage of S.aureus in clinical mastitis was (28.2%) , while in in subclinical mastitis was (22.46% ).The identification of S,aureus was confirmed by PCR amplification of species-specific parts of the gene encoding the 16S rRNA. Twenty nine S.aureus isolates were subjected to PCR for detection of X region of protein A (spA) gene,10 isolates from clinical mastitis and 19 isolates subclinical mastitiC milk specimens (+++ve CMT and ++ve CMT) were (100%) positive . Results indicate that there was a positive relationship between the presence of protein A gene of S.aureus and the inflammation of udder in clinical mastitis as well as the high grades of subclinical mastitis. | ||
| Keywords | ||
| Phenotypic; genotypic; S. aureus; bovine; mastitis | ||
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