Seroprevalence of Camel (Camelus dromedarius) Trypanosomiasis, with special Reference to Gene Sequencing of Trypanosoma evansi in Sharkia Governorate
Elbalkemy, F., Menazi, A., Selim, A., Wahba, A., El-Shazly, Y. (2016). Seroprevalence of Camel (Camelus dromedarius) Trypanosomiasis, with special Reference to Gene Sequencing of Trypanosoma evansi in Sharkia Governorate. EKB Journal Management System, 44(3), 187-195. doi: 10.21608/zvjz.2016.7871
Farouk Elbalkemy; Afaf Menazi; Adballah Selim; Ahmed Wahba; Yousry El-Shazly. "Seroprevalence of Camel (Camelus dromedarius) Trypanosomiasis, with special Reference to Gene Sequencing of Trypanosoma evansi in Sharkia Governorate". EKB Journal Management System, 44, 3, 2016, 187-195. doi: 10.21608/zvjz.2016.7871
Elbalkemy, F., Menazi, A., Selim, A., Wahba, A., El-Shazly, Y. (2016). 'Seroprevalence of Camel (Camelus dromedarius) Trypanosomiasis, with special Reference to Gene Sequencing of Trypanosoma evansi in Sharkia Governorate', EKB Journal Management System, 44(3), pp. 187-195. doi: 10.21608/zvjz.2016.7871
Elbalkemy, F., Menazi, A., Selim, A., Wahba, A., El-Shazly, Y. Seroprevalence of Camel (Camelus dromedarius) Trypanosomiasis, with special Reference to Gene Sequencing of Trypanosoma evansi in Sharkia Governorate. EKB Journal Management System, 2016; 44(3): 187-195. doi: 10.21608/zvjz.2016.7871

Seroprevalence of Camel (Camelus dromedarius) Trypanosomiasis, with special Reference to Gene Sequencing of Trypanosoma evansi in Sharkia Governorate

Zagazig Veterinary Journal
Article 1, Volume 44, Issue 3, December 2016, Page 187-195  XML PDF (521.41 K)
Document Type: Original Article
DOI: 10.21608/zvjz.2016.7871
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Authors
Farouk Elbalkemy1; Afaf Menazi1; Adballah Selim1; Ahmed Wahba2; Yousry El-Shazly email 3
1Animal Medicine Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
2Animal Health Research Institute, Dokki, Giza, Egypt
3The Veterinary Clinic, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt
Abstract
A total of 330 camels (Camelus dromedarius) of both sexes and with different ages, at different localities in Sharkia Governorate, Egypt, were examined clinically from April 2013 to March 2015. Some adult camels showed emaciation, edema of legs and abdomen which were suspected to be infected by Trypanosoma species. This study aimed to determine the seroprevalence of trypanosomiasis using indirect ELISA test and the results showed that 174 (52.7%) were infected. The infection rate was higher in males (54.8%) than females (42.2%), also, in camels more than 2-years old and in summer and spring seasons. Thirty blood samples from the examined animals by ELISA test (15 positive and 15 negative) were subjected to PCR. The results revealed that 18 samples were positive for T. evansi by PCR. Two positive samples for RoTat1.2 VSG encoding gene were chosen for DNA sequence analysis, one of them was obtained from newly imported camel in a camel farm at Belbais city and the other one belonged to a camel from one of Belbais villages at Sharkia Governorate. Nucleotide sequence alignment of RoTat1.2 VSG gene variants from Egyptian T. evansi showed some heterogeneity with other T. evansi isolates from Egypt. In conclusion, PCR technique is more sensitive and specific than ELISA for the diagnosis of T. evansi infection in camels.
Keywords
camels; Trypanosoma evansi; seroprevalence; ELISA
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