Modified Method for Propagation of Rift Valley Fever Virus | ||||
| Egyptian Journal of Veterinary Sciences | ||||
| Article 4, Volume 46, Issue 1, September 2015, Page 33-40 PDF (374.19 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejvs.2015.848 | ||||
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| Abstract | ||||
| Trials for propagation of RVFV on Vero cells using different MOI 0.1, 0.01 & 0.001 and different concentrations of trypsin (25 & 50 µg/ml). The result indicated that high titer of RVFV (8.0 log10 TCID50/ml in Vero cells, 7.5 log10 MIPLD50/ml in adult mice and 7.7 log10 MICLD50/ml in baby mice) was obtained with MOI 0.001 using 50 µg / ml of trypsin after 24 hrs post inoculation. Inoculation of freshly subcultured Vero cells as other method lead to obtaining high virus titer (8.5 log10 TCID50/ml in Vero cells, 7.6 log10 MIPLD50/ml in adult mice and 8.2 log10 MICLD50/ml in baby mice) after 48 hours with MOI 0.0001. While using subculture of monolayer Vero cells 24 hours previously infected with RVFV, it was obtained high titer (8.0 log10 TCID50/ml in Vero cells, 7.4 log10 MIPLD50 / ml in adult mice and 7.9 log10 MICLD50/ml in baby mice) with MOI 0.001 as well as a triple amount of virus yielded than the traditional method. Different batches of inactivated RVFV vaccine were prepared from the harvested virus which was identified as RVFV using AGPT. Quality control tests were applied proved that all vaccine batches were sterile, safe and potent. It is concluded that modified methods could increase RVFV production with high titer as well as saving time, saving materials used in virus production include quantity of inoculums. | ||||
| Keywords | ||||
| Rift valley; RVFV; Vero cells; vaccine | ||||
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