EFFECT OF LINEZOLID ALONE AND IN COMBINATION WITH OTHER ANTIBIOTICS, ON METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS
YEHIA, H., EL SAID, M., AZMY, M., BADAWY, M., MANSY, S., GOHAR, H., MADANY, N. (2016). EFFECT OF LINEZOLID ALONE AND IN COMBINATION WITH OTHER ANTIBIOTICS, ON METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS. EKB Journal Management System, 46(1), 57-66. doi: 10.21608/jesp.2016.88935
HODA YEHIA; MANAL EL SAID; MAGDA AZMY; MOUSHIRA BADAWY; SOHEIR MANSY; HAMIDA GOHAR; NADIA MADANY. "EFFECT OF LINEZOLID ALONE AND IN COMBINATION WITH OTHER ANTIBIOTICS, ON METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS". EKB Journal Management System, 46, 1, 2016, 57-66. doi: 10.21608/jesp.2016.88935
YEHIA, H., EL SAID, M., AZMY, M., BADAWY, M., MANSY, S., GOHAR, H., MADANY, N. (2016). 'EFFECT OF LINEZOLID ALONE AND IN COMBINATION WITH OTHER ANTIBIOTICS, ON METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS', EKB Journal Management System, 46(1), pp. 57-66. doi: 10.21608/jesp.2016.88935
YEHIA, H., EL SAID, M., AZMY, M., BADAWY, M., MANSY, S., GOHAR, H., MADANY, N. EFFECT OF LINEZOLID ALONE AND IN COMBINATION WITH OTHER ANTIBIOTICS, ON METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS. EKB Journal Management System, 2016; 46(1): 57-66. doi: 10.21608/jesp.2016.88935

EFFECT OF LINEZOLID ALONE AND IN COMBINATION WITH OTHER ANTIBIOTICS, ON METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS

Journal of the Egyptian Society of Parasitology
Article 7, Volume 46, Issue 1, April 2016, Page 57-66  XML PDF (4.59 MB)
Document Type: Original Article
DOI: 10.21608/jesp.2016.88935
View on SCiNiTO View on SCiNiTO
Authors
HODA YEHIA1; MANAL EL SAID2; MAGDA AZMY1; MOUSHIRA BADAWY1; SOHEIR MANSY1; HAMIDA GOHAR3; NADIA MADANY3
1Department of Electron Microscopy, Theodor Bilharz Research Institute, Imbaba, PO Box: 30, Giza, 12411, Egypt.
2Department of Microbiology, Theodor Bilharz Research Institute, Imbaba, PO Box: 30, Giza, 12411, Egypt.
3Faculty of Medicine, Cairo University, Egypt.
Abstract
The prevalence of methicillin-resistant Staphyloccoccus aureus (MRSA) strains has presented a new challenge in antimicrobial medication. Linezolid is a new drug with potent activity on Grampositive pathogens such as MRSA. The aim of the study was to investigate the in vitro activity of linezolid alone and in combination with imipenem, vancomycin or rifampicin to determine the most active therapy against MRSA strains. Twenty clinical MRSA strains were isolated from patients admitted to inpatient departments and outpatient clinics of Theodor Bilharz Research Institute. Standard strain MRSA ATCC 43300 was included as a control. The MICs of MRSA strains to linezolid, vancomycin, imipenem and rifampicin were evaluated using E test. Time-kill curve were used to assess the in vitro activity of linezolid (at 8x MIC) alone and in combination with imipenem (at 32x MIC), vancomycin or rifampicin (at 8x MIC). Scanning and transmission electron microscopy were performed to compare bacterial morphological alterations owing to the different combinations.
Time-kill studies showed synergistic effect when linezolid combined with imipenem was tested against all the MRSA strains. Linezolid plus vancomycin or rifampicin combinations did not display any synergism or antagonism. Scanning and transmission electron microscopy observations confirmed the interactions observed in time kill experiments. Linezolid in combination with subinhibitory concentrations of imipenem can be bactericidal against MRSA strains and appears to be a
promising combination for the treatment of MRSA infections. No synergistic activity was seen when the linezolid and vancomycin or rifampicin were combined. Linezolid could prevent the emergence of mutants resistant to rifampicin
Keywords
MRSA; linezolid; time kill; electron microscopy
Statistics
Article View: 159
PDF Download: 224