DETECTION OF Brucella melitensis FROM FILTERED BOVINE PERIPHERAL-BLOOD BY POLYMERASE CHAIN REACTION TEST | ||
| Journal of Agricultural Chemistry and Biotechnology | ||
| Article 3, Volume 1, Issue 4, April 2010, Pages 229-234 PDF (347.8 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jacb.2010.89424 | ||
| Authors | ||
| Buthaina Al-Ashkar1; A. Al-Mariri2; Ibtissam Hamad3 | ||
| 1Dept. of Plant Biology, Faculty of Science, University of Damascus | ||
| 2Dept. of Molecular Biology and Biotechnology, Atomic Energy Commission, Damascus | ||
| 3Dept. of Environment, Faculty of Science, University of Damascus | ||
| Abstract | ||
| Brucella melitensis contaminants of bovine peripheral-blood can be concentrated and isolated by filtration; and their DNA can be released for subsequent PCR detection by heating the isolated colony at 95°C for 10 min. Detecting rate of brucellosis in the infected sample was less than 20 CFU/ml of blood, after leukocytes filtration and culturing, as well as on isolated colonies. This PCR method also enables us to make the diagnosing of brucellosis for the animals with a negative complement fixation test. | ||
| Keywords | ||
| Blood; Brucella melitensis; Leukocytes; PCR | ||
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