COMPARISON BETWEEN ELISA AND VARIOUS STAINS TECHNIQUES IN LABORATORY DIAGNOSIS OF CRYPTOSPORIDIOSIS | ||
| Journal of the Egyptian Society of Parasitology | ||
| Article 23, Volume 44, Issue 2, August 2014, Pages 509-516 PDF (241.56 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jesp.2014.90410 | ||
| Authors | ||
| NABIL S. GABR; MANAL Z. M. ABDELLATIF; EKHLAS H. ABD EL-HAFEEZ; REHAM A. M. ABD RABOU | ||
| Department of Parasitology, Faculty of Medicine, Minia University, Minia, 61519, Egypt. | ||
| Abstract | ||
| Cryptosporidium spp. is an important parasitic protozoa causing diarrhea which is a severe life-threatening diarrhea especially in immunocompromised hosts. We aimed to evaluate the usefulness of detection of Cryptosporidium spp. copro-antigen from fecal specimens by using enzyme linked immunosorbent assay (ELISA) test and comparing its sensitivity and specificity with some staining methods. The results revealed that Modified Acid-Fast stain is considered better than Giemsa in detecting Cryptosporidium species oocysts in faecal smears as their sensitivity were 67.5% and 53.75% respectively. On contrary, ELISA technique is considered the best method used for detection of cryptosporidial infection as its sensitivity is 90%. | ||
| Keywords | ||
| Egypt; Cryptosporidium parvum; persistent diarrhea and ELISA technique | ||
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